High glucose levels affect retinal patterning during zebrafish embryogenesis

Amitoj Singh, Hozana Andrade Castillo, Julie Brown, Jan Kaslin, Karen M. Dwyer, Yann Gibert

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Maternal hyperglycaemia has a profound impact on the developing foetus and increases the risk of developing abnormalities like obesity, impaired glucose tolerance and insulin secretory defects in the post-natal life. Increased levels of glucose in the blood stream due to diabetes causes visual disorders like retinopathy. However, the impact of maternal hyperglycaemia due to pre-existing or gestational diabetes on the developing foetal retina is unknown. The aim of this work was to study the effect of hyperglycaemia on the developing retina using zebrafish as a vertebrate model. Wild-type and transgenic zebrafish embryos were exposed to 0, 4 and 5% D-Glucose in a pulsatile manner to mimic the fluctuations in glycaemia experienced by the developing foetus in pregnant women with diabetes. The zebrafish embryos displayed numerous ocular defects associated with altered retinal cell layer thickness, increased presence of macrophages, and decreased number of Müeller glial and retinal ganglion cells following high-glucose exposure. We have developed a model of gestational hyperglycaemia using the zebrafish embryo to study the effect of hyperglycaemia on the developing embryonic retina. The data suggests that glucose exposure is detrimental to the development of embryonic retina and the legacy of this exposure may extend into adulthood. These data suggest merit in retinal assessment in infants born to mothers with pre-existing and gestational diabetes both in early and adult life.

Original languageEnglish
Article number4121
Number of pages13
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 11 Mar 2019

Keywords

  • disease model
  • embryology

Cite this

Singh, Amitoj ; Castillo, Hozana Andrade ; Brown, Julie ; Kaslin, Jan ; Dwyer, Karen M. ; Gibert, Yann. / High glucose levels affect retinal patterning during zebrafish embryogenesis. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Maternal hyperglycaemia has a profound impact on the developing foetus and increases the risk of developing abnormalities like obesity, impaired glucose tolerance and insulin secretory defects in the post-natal life. Increased levels of glucose in the blood stream due to diabetes causes visual disorders like retinopathy. However, the impact of maternal hyperglycaemia due to pre-existing or gestational diabetes on the developing foetal retina is unknown. The aim of this work was to study the effect of hyperglycaemia on the developing retina using zebrafish as a vertebrate model. Wild-type and transgenic zebrafish embryos were exposed to 0, 4 and 5{\%} D-Glucose in a pulsatile manner to mimic the fluctuations in glycaemia experienced by the developing foetus in pregnant women with diabetes. The zebrafish embryos displayed numerous ocular defects associated with altered retinal cell layer thickness, increased presence of macrophages, and decreased number of M{\"u}eller glial and retinal ganglion cells following high-glucose exposure. We have developed a model of gestational hyperglycaemia using the zebrafish embryo to study the effect of hyperglycaemia on the developing embryonic retina. The data suggests that glucose exposure is detrimental to the development of embryonic retina and the legacy of this exposure may extend into adulthood. These data suggest merit in retinal assessment in infants born to mothers with pre-existing and gestational diabetes both in early and adult life.",
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High glucose levels affect retinal patterning during zebrafish embryogenesis. / Singh, Amitoj; Castillo, Hozana Andrade; Brown, Julie; Kaslin, Jan; Dwyer, Karen M.; Gibert, Yann.

In: Scientific Reports, Vol. 9, No. 1, 4121, 11.03.2019.

Research output: Contribution to journalArticleResearchpeer-review

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