High-efficiency pharmacogenetic ablation of oligodendrocyte progenitor cells in the adult mouse CNS

Yao Lulu Xing, Jasmine Poh, Bernard H.A. Chuang, Kaveh Moradi, Stanislaw Mitew, William D. Richardson, Trevor J. Kilpatrick, Yasuyuki Osanai, Tobias D. Merson

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2 Citations (Scopus)


Approaches to investigate adult oligodendrocyte progenitor cells (OPCs) by targeted cell ablation in the rodent CNS have limitations in the extent and duration of OPC depletion. We have developed a pharmacogenetic approach for conditional OPC ablation, eliminating >98% of OPCs throughout the brain. By combining recombinase-based transgenic and viral strategies for targeting OPCs and ventricular-subventricular zone (V-SVZ)-derived neural precursor cells (NPCs), we found that new PDGFRA-expressing cells born in the V-SVZ repopulated the OPC-deficient brain starting 12 days after OPC ablation. Our data reveal that OPC depletion induces V-SVZ-derived NPCs to generate vast numbers of PDGFRA+NG2+ cells with the capacity to proliferate and migrate extensively throughout the dorsal anterior forebrain. Further application of this approach to ablate OPCs will advance knowledge of the function of both OPCs and oligodendrogenic NPCs in health and disease.

Original languageEnglish
Article number100414
Number of pages21
JournalCell Reports Methods
Issue number2
Publication statusPublished - 27 Feb 2023


  • cell ablation
  • CP: neuroscience
  • diphtheria toxin
  • neural precursor cells
  • neural stem cells
  • NG2 glia
  • oligodendrocyte progenitor cells
  • oligodendrocytes
  • regeneration
  • ventricular-subventricular zone

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