High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats

Ahmed Regina; Anthony Bird; David Topping; Sarah Bowden; Judy Freeman; Tina Barsby; Behjat Kosar-Hashemi; Zhongyi Li; Sadequr Rahman; Matthew Morell

doi:10.1073/pnas.0510737103

High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats

Ahmed Regina, Anthony Bird, David Topping, Sarah Bowden, Judy Freeman, Tina Barsby, Behjat Kosar-Hashemi, Zhongyi Li, Sadequr Rahman, Matthew Morell

Research output: Contribution to journal › Article › Research › peer-review

494 Citations (Scopus)

Abstract

Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

Original language	English
Pages (from-to)	3546-3551
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	103
Issue number	10
DOIs	https://doi.org/10.1073/pnas.0510737103
Publication status	Published - 7 Mar 2006
Externally published	Yes

Keywords

Genetic engineering
Nutrition
Starch

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.0510737103

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title = "High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats",

abstract = "Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70\% amylose. When the >70\% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.",

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author = "Ahmed Regina and Anthony Bird and David Topping and Sarah Bowden and Judy Freeman and Tina Barsby and Behjat Kosar-Hashemi and Zhongyi Li and Sadequr Rahman and Matthew Morell",

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Regina, A, Bird, A, Topping, D, Bowden, S, Freeman, J, Barsby, T, Kosar-Hashemi, B, Li, Z, Rahman, S & Morell, M 2006, 'High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats', Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 10, pp. 3546-3551. https://doi.org/10.1073/pnas.0510737103

TY - JOUR

T1 - High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats

AU - Regina, Ahmed

AU - Bird, Anthony

AU - Topping, David

AU - Bowden, Sarah

AU - Freeman, Judy

AU - Barsby, Tina

AU - Kosar-Hashemi, Behjat

AU - Li, Zhongyi

AU - Rahman, Sadequr

AU - Morell, Matthew

PY - 2006/3/7

Y1 - 2006/3/7

N2 - Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

AB - Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

KW - Genetic engineering

KW - Nutrition

KW - Starch

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U2 - 10.1073/pnas.0510737103

DO - 10.1073/pnas.0510737103

M3 - Article

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AN - SCOPUS:33644871347

SN - 0027-8424

VL - 103

SP - 3546

EP - 3551

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 10

ER -

High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats

Abstract

Keywords

UN SDGs

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