Hierarchical drug release of pH-sensitive liposomes encapsulating aqueous two phase system

Xunan Zhang, Wei Zong, Hongmei Bi, Kunming Zhao, Thomas Fuhs, Ying Hu, Wenlong Cheng, Xiaojun Han

Research output: Contribution to journalArticleResearchpeer-review

Abstract

As promising drug delivery vehicles, previous investigations of liposomes as carriers are primarily focused on insertion and modification of lipid membrane interfaces. The utility of the inner core seems to be overlooked. Herein, we developed pH-sensitive liposomes (PSLs) containing an aqueous two phase system (ATPS), and intriguingly discovered their hierarchical release under acidic stimuli. ATPS containing two polymers (poly(ethylene glycol) (PEG) and dextran) is homogeneous above phase transition temperature when producing ATPS-liposomes, and separated into PEG-rich phase and dextran-rich phase after cooling down to room temperature. The overall release time of ATPS-liposomes is divided into two stages and prolonged compared to simple aqueous liposomes. The unique release profile is due to the disproportional distribution of drugs in two phases. Doxorubicin (DOX) is loaded in the ATPS-liposomes, and their half maximum inhibition concentration on HeLa cells is 0.018 μmol L−1, which means 27.5 fold increase in inhibition efficiency over free DOX.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Volume127
DOIs
Publication statusPublished - 1 Jun 2018

Keywords

  • Aqueous two phase system (ATPS)
  • Hierarchical drug release
  • Liposome

Cite this

Zhang, Xunan ; Zong, Wei ; Bi, Hongmei ; Zhao, Kunming ; Fuhs, Thomas ; Hu, Ying ; Cheng, Wenlong ; Han, Xiaojun. / Hierarchical drug release of pH-sensitive liposomes encapsulating aqueous two phase system. In: European Journal of Pharmaceutics and Biopharmaceutics. 2018 ; Vol. 127. pp. 177-182.
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Hierarchical drug release of pH-sensitive liposomes encapsulating aqueous two phase system. / Zhang, Xunan; Zong, Wei; Bi, Hongmei; Zhao, Kunming; Fuhs, Thomas; Hu, Ying; Cheng, Wenlong; Han, Xiaojun.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 127, 01.06.2018, p. 177-182.

Research output: Contribution to journalArticleResearchpeer-review

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