Hic-5 affects proliferation, migration and invasion of B16 murine melanoma cells

Fumihito Noguchi, Shigeki Inui, Takeshi Nakajima, Satoshi Itami

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8 Citations (Scopus)


Hic-5 is a shuttling protein between the cell membrane and the nucleus which functions as a focal adhesion adaptor protein and a nuclear receptor coactivator. Although several studies have shown its involvement in other types of cancer, the role of Hic-5 in melanoma is unknown. Herein, we show for the first time that Hic-5 is expressed in B16-F1 murine melanoma cells. To determine its function in melanoma cells, we used shRNA-mediated RNA interference and established stable clones with down-regulated Hic-5 expression. These clones had impaired growth and metastatic potential compared with controls in vivo, which correlated with decreased proliferation, migration and invasion in vitro. Moreover, silencing of Hic-5 expression in B16-F1 activated RhoA with an amoeboid phenotypic change, indicating that Hic-5 is a key regulator of B16-F1 metastasis in the context of Rho-dependent motility. These results provide new evidence that Hic-5 is a possible molecular target for treatment of melanoma.

Original languageEnglish
Pages (from-to)773-782
Number of pages10
JournalPigment Cell and Melanoma Research
Issue number6
Publication statusPublished - 1 Nov 2012
Externally publishedYes


  • Hic5 protein
  • Melanoma
  • Mouse
  • Neoplasm metastasis
  • RhoA GTP-binding protein

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