Heterozygous expression of the oncogenic Pik3ca (H1047R) mutation during murine development results in fatal embryonic and extraembryonic defects

Lauren M Hare, Quenten Philip Schwarz, Sophie Wiszniak, Rajendra Gurung, Karen G Montgomery, Christina Anne Mitchell, Wayne A Phillips

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

The phosphoinositide 3-kinase (PI3K)/AKT signalling pathway regulates many cellular functions including proliferation, migration, survival and protein synthesis. Somatic mutations in PIK3CA, the gene encoding the p110alpha catalytic subunit of PI3K enzyme, are commonly associated with many human cancers as well as recently being implicated in human overgrowth syndromes. However, it is not clear if such mutations can be inherited through the germline. We have used a novel mouse model with Cre recombinase (Cre)-conditional knock-in of the common H1047R mutation into the endogenous Pik3ca gene. Heterozygous expression of the Pik3caH1047R mutation in the developing mouse embryo resulted in failed turning of the embryo and disrupted vascular remodelling within the embryonic and extraembryonic tissues, leading to lethality prior to E10. As vascular endothelial growth factor A (VEGF-A) signalling was disrupted in these embryos, we used Cre under the control of the Tie2 promoter to target the Pik3caH1047R mutation specifically to endothelial cells. In these embryos turning occurred normally but the vascular remodelling defects and embryonic lethality remained, likely as a result of endothelial hyperproliferation. Our results confirm the lethality associated with heterozygous expression of the Pik3caH1047R mutation during development and likely explain the lack of inherited germline PIK3CA mutations in humans.
Original languageEnglish
Pages (from-to)14-26
Number of pages13
JournalDevelopmental Biology
Volume404
Issue number1
DOIs
Publication statusPublished - 1 Aug 2015

Cite this