Hepatic TLR2 & TLR4 expression correlates with hepatic inflammation and TNF-a in HCV & HCV/HIV infection

Mark Berzsenyi, Stuart Roberts, Scott Preiss, David Woollard, Michael Beard, Narelle Skinner, David Bowden, Kumar Visvanathan

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40 Citations (Scopus)

Abstract

Signalling activated by Toll-like receptors (TLRs) can result in the production of tumour necrosis factor alpha (TNF-alpha) which is implicated in hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infection. No study has examined or compared hepatic expression of TLRs in both HCV and HCV/HIV. Liver and peripheral blood mononuclear cells (PBMCs) were obtained from HCV HCV/HIV-infected patients and PBMCs from HIV-infected patients. Liver RNA was analysed by microarray and reverse transcription quantitative PCR (RT-qPCR). PBMCs were analysed by flow cytometry. Associations with hepatic histology and infection type were sought. Forty-six HCV, 20 HIV and 27 HCV/HIV-infected patients were recruited. Increasing Metavir inflammatory activity score was associated with increased hepatic TLR mRNA by RT-qPCR: TLR2 (P
Original languageEnglish
Pages (from-to)852 - 860
Number of pages9
JournalJournal of Viral Hepatitis
Volume18
Issue number12
DOIs
Publication statusPublished - 2011

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