Heater integrated lab‐on‐a‐chip device for rapid hla alleles amplification towards prevention of drug hypersensitivity

Shah Mukim Uddin, Abkar Sayad, Jianxiong Chan, Duc Hau Huynh, Efstratios Skafidas, Patrick Kwan

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

HLA‐B*15:02 screening before administering carbamazepine is recommended to prevent life‐threatening hypersensitivity. However, the unavailability of a point‐of‐care device impedes this screening process. Our research group previously developed a two‐step HLA‐B*15:02 detection technique utilizing loop‐mediated isothermal amplification (LAMP) on the tube, which requires two‐stage device development to translate into a portable platform. Here, we report a heaterintegrated lab‐on‐a‐chip device for the LAMP amplification, which can rapidly detect HLA‐B alleles colorimetrically. A gold‐patterned micro‐sized heater was integrated into a 3D‐printed chip, allowing microfluidic pumping, valving, and incubation. The performance of the chip was tested with color dye. Then LAMP assay was conducted with human genomic DNA samples of known HLA‐B genotypes in the LAMP‐chip parallel with the tube assay. The LAMP‐on‐chip results showed a complete match with the LAMP‐on‐tube assay, demonstrating the detection system’s concurrence.

Original languageEnglish
Article number3413
Number of pages14
JournalSensors
Volume21
Issue number10
DOIs
Publication statusPublished - 2 May 2021

Keywords

  • Lab‐on‐a‐chip
  • Loop‐mediated isothermal amplification
  • Microfluidics
  • Pharmaco‐genetics
  • Point‐of‐care diagnostics

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