TY - JOUR
T1 - Haloalkaliphilic microorganisms assist sulfide removal in a microbial electrolysis cell
AU - Ni, Gaofeng
AU - Harnawan, Pebrianto
AU - Seidel, Laura
AU - Ter Heijne, Annemiek
AU - Sleutels, Tom
AU - Buisman, Cees J.N.
AU - Dopson, Mark
N1 - Funding Information:
This work was performed within the cooperation framework of the Linnaeus University Centre for Ecology and Evolution in Microbial Model Systems (EEMiS) and Wetsus, European Centre of Excellence for Sustainable Water Technology. The authors thank Martijn Bijmans for contributing to the experimental design and Daniel Lundin for assistance in bioinformatic analysis. The authors also thank Paweł Roman, Pau Rodenas Motos, Casper Borsje, Sam Molenaar, Yang Lei, Philipp Kuntke, Karine Kiragosyan, Harm van der Kooi, Mieke Kersaan-Haan, Ton van de Zande, and Ilse Gerrits for useful discussions and analytical assistance. The authors acknowledge support from Science for Life Laboratory and the National Genomics Infrastructure for providing assistance in massive parallel sequencing and computational infrastructure. The computations were performed on resources provided by SNIC through Uppsala Multidisciplinary Center for Advanced Computational Science (UPPMAX) under Project snic2017-7-182.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/2/5
Y1 - 2019/2/5
N2 - Several industrial processes produce toxic sulfide containing streams that are often scrubbed using caustic solutions. An alternative, cost effective sulfide treatment method is bioelectrochemical sulfide removal. For the first time, a haloalkaliphilic sulfide-oxidizing microbial consortium was introduced to the anodic chamber of a microbial electrolysis cell operated at alkaline pH and with 1.0 M sodium ions. Under anode potential control, the highest sulfide removal rate was 2.16 mM/day and chemical analysis supported that the electrical current generation was from the sulfide oxidation. Biotic operation produced a maximum current density of 3625 mA/m2 compared to 210 mA/m2 while under abiotic operation. Furthermore, biotic electrical production was maintained for a longer period than for abiotic operation, potentially due to the passivation of the electrode by elemental sulfur during abiotic operation. The use of microorganisms reduced the energy input in this study compared to published electrochemical sulfide removal technologies. Sulfide-oxidizing populations dominated both the planktonic and electrode-attached communities with 16S rRNA gene sequences aligning within the genera Thioalkalivibrio, Thioalkalimicrobium, and Desulfurivibrio. The dominance of the Desulfurivibrio-like population on the anode surface offered evidence for the first haloalkaliphilic bacterium able to couple electrons from sulfide oxidation to extracellular electron transfer to the anode.
AB - Several industrial processes produce toxic sulfide containing streams that are often scrubbed using caustic solutions. An alternative, cost effective sulfide treatment method is bioelectrochemical sulfide removal. For the first time, a haloalkaliphilic sulfide-oxidizing microbial consortium was introduced to the anodic chamber of a microbial electrolysis cell operated at alkaline pH and with 1.0 M sodium ions. Under anode potential control, the highest sulfide removal rate was 2.16 mM/day and chemical analysis supported that the electrical current generation was from the sulfide oxidation. Biotic operation produced a maximum current density of 3625 mA/m2 compared to 210 mA/m2 while under abiotic operation. Furthermore, biotic electrical production was maintained for a longer period than for abiotic operation, potentially due to the passivation of the electrode by elemental sulfur during abiotic operation. The use of microorganisms reduced the energy input in this study compared to published electrochemical sulfide removal technologies. Sulfide-oxidizing populations dominated both the planktonic and electrode-attached communities with 16S rRNA gene sequences aligning within the genera Thioalkalivibrio, Thioalkalimicrobium, and Desulfurivibrio. The dominance of the Desulfurivibrio-like population on the anode surface offered evidence for the first haloalkaliphilic bacterium able to couple electrons from sulfide oxidation to extracellular electron transfer to the anode.
KW - 16S rRNA gene amplicon sequencing
KW - Bioelectrochemical systems
KW - Desulfurivibrio
KW - Sulfides
UR - http://www.scopus.com/inward/record.url?scp=85054420208&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2018.09.049
DO - 10.1016/j.jhazmat.2018.09.049
M3 - Article
C2 - 30308358
AN - SCOPUS:85054420208
SN - 0304-3894
VL - 363
SP - 197
EP - 204
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
ER -