TY - JOUR
T1 - H-score of 11β-hydroxylase and aldosterone synthase in the histopathological diagnosis of adrenocortical tumors
AU - Yang, Yi
AU - Xiao, Ming
AU - Song, Ying
AU - Tang, Yi
AU - Luo, Ting
AU - Yang, Shumin
AU - He, Wenwen
AU - Cheng, Qingfeng
AU - Ma, Linqiang
AU - Zhang, Yao
AU - He, Yunfeng
AU - Cao, Youde
AU - Yang, Jun
AU - Peng, Bin
AU - Hu, Jinbo
AU - Li, Qifu
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Purpose: To assess the diagnostic performance of the H-score of 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) in the histopathological diagnosis of adrenocortical tumors (ACT). Methods: We retrospectively evaluated 199 cases of ACT, of which 85 were diagnosed as aldosterone-producing adenoma (APA), 66 as cortisol-producing adenoma (CPA), 9 as aldosterone–cortisol co-secreting adenoma, 30 as nonhyperfunctioning adenoma, and 9 as adrenocortical carcinoma (ACC). Immunohistochemical staining was performed using anti-CYP11B1 and anti-CYP11B2 monoclonal antibodies. The staining was quantified by the McCarty’s H-score system. The diagnostic performance was assessed by the receiver operating characteristic curve (ROC). Results: The H-score of CYP11B1 is highest in the CPA group and lowest in the ACC group. The H-score of CYP11B2 in the APA group is significantly higher than other ACT groups. The area under ROC (AUC) of an increased H-score of CYP11B2 (>65) for the diagnosis of APA was 0.971 (95%CI 0.937–0.990). The AUC of an increased H-score of CYP11B1 (>204) for the diagnosis of CPA was 0.725 (95%CI 0.658–0.786). The AUC of a decreased H-score of CYP11B1 (<85) for the diagnosis of ACC was 0.960 (95%CI 0.923–0.983). Conclusions: H-score of CYP11B1 and CYP11B2 are reliable tools for the histopathological subtyping of functional benign ACT and may offer some value in the histopathological diagnosis of malignant ACT.
AB - Purpose: To assess the diagnostic performance of the H-score of 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) in the histopathological diagnosis of adrenocortical tumors (ACT). Methods: We retrospectively evaluated 199 cases of ACT, of which 85 were diagnosed as aldosterone-producing adenoma (APA), 66 as cortisol-producing adenoma (CPA), 9 as aldosterone–cortisol co-secreting adenoma, 30 as nonhyperfunctioning adenoma, and 9 as adrenocortical carcinoma (ACC). Immunohistochemical staining was performed using anti-CYP11B1 and anti-CYP11B2 monoclonal antibodies. The staining was quantified by the McCarty’s H-score system. The diagnostic performance was assessed by the receiver operating characteristic curve (ROC). Results: The H-score of CYP11B1 is highest in the CPA group and lowest in the ACC group. The H-score of CYP11B2 in the APA group is significantly higher than other ACT groups. The area under ROC (AUC) of an increased H-score of CYP11B2 (>65) for the diagnosis of APA was 0.971 (95%CI 0.937–0.990). The AUC of an increased H-score of CYP11B1 (>204) for the diagnosis of CPA was 0.725 (95%CI 0.658–0.786). The AUC of a decreased H-score of CYP11B1 (<85) for the diagnosis of ACC was 0.960 (95%CI 0.923–0.983). Conclusions: H-score of CYP11B1 and CYP11B2 are reliable tools for the histopathological subtyping of functional benign ACT and may offer some value in the histopathological diagnosis of malignant ACT.
KW - 11β-hydroxylase
KW - Adrenocortical Tumors
KW - Aldosterone synthase
KW - H-score
KW - Histopathological diagnosis
UR - http://www.scopus.com/inward/record.url?scp=85069530207&partnerID=8YFLogxK
U2 - 10.1007/s12020-019-02022-8
DO - 10.1007/s12020-019-02022-8
M3 - Article
C2 - 31332713
AN - SCOPUS:85069530207
SN - 1355-008X
VL - 65
SP - 683
EP - 691
JO - Endocrine
JF - Endocrine
IS - 3
ER -