Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

Andrea Cossarizza, Hyun Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W. Agace, Nima Aghaeepour, Mübeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrä, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T. Baldari, Sudipto Bari, Vincenzo BarnabaJessica G. Borger, Martin S. Davey, Kylie Quinn, Anouk Von Borstel

Research output: Contribution to journalArticleOtherpeer-review

30 Citations (Scopus)

Abstract

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
Original languageEnglish
Pages (from-to)1457-1973
Number of pages517
JournalEuropean Journal of Immunology
Volume49
Issue number10
DOIs
Publication statusPublished - 1 Oct 2019

Cite this

Cossarizza, Andrea ; Chang, Hyun Dong ; Radbruch, Andreas ; Acs, Andreas ; Adam, Dieter ; Adam-Klages, Sabine ; Agace, William W. ; Aghaeepour, Nima ; Akdis, Mübeccel ; Allez, Matthieu ; Almeida, Larissa Nogueira ; Alvisi, Giorgia ; Anderson, Graham ; Andrä, Immanuel ; Annunziato, Francesco ; Anselmo, Achille ; Bacher, Petra ; Baldari, Cosima T. ; Bari, Sudipto ; Barnaba, Vincenzo ; Borger, Jessica G. ; Davey, Martin S. ; Quinn, Kylie ; Von Borstel, Anouk. / Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). In: European Journal of Immunology. 2019 ; Vol. 49, No. 10. pp. 1457-1973.
@article{dbda6fc26dbd48d99e90732b27905c35,
title = "Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)",
abstract = "These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.",
author = "Andrea Cossarizza and Chang, {Hyun Dong} and Andreas Radbruch and Andreas Acs and Dieter Adam and Sabine Adam-Klages and Agace, {William W.} and Nima Aghaeepour and M{\"u}beccel Akdis and Matthieu Allez and Almeida, {Larissa Nogueira} and Giorgia Alvisi and Graham Anderson and Immanuel Andr{\"a} and Francesco Annunziato and Achille Anselmo and Petra Bacher and Baldari, {Cosima T.} and Sudipto Bari and Vincenzo Barnaba and Borger, {Jessica G.} and Davey, {Martin S.} and Kylie Quinn and {Von Borstel}, Anouk",
year = "2019",
month = "10",
day = "1",
doi = "10.1002/eji.201970107",
language = "English",
volume = "49",
pages = "1457--1973",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "10",

}

Cossarizza, A, Chang, HD, Radbruch, A, Acs, A, Adam, D, Adam-Klages, S, Agace, WW, Aghaeepour, N, Akdis, M, Allez, M, Almeida, LN, Alvisi, G, Anderson, G, Andrä, I, Annunziato, F, Anselmo, A, Bacher, P, Baldari, CT, Bari, S, Barnaba, V, Borger, JG, Davey, MS, Quinn, K & Von Borstel, A 2019, 'Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)', European Journal of Immunology, vol. 49, no. 10, pp. 1457-1973. https://doi.org/10.1002/eji.201970107

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). / Cossarizza, Andrea; Chang, Hyun Dong; Radbruch, Andreas; Acs, Andreas; Adam, Dieter; Adam-Klages, Sabine; Agace, William W.; Aghaeepour, Nima; Akdis, Mübeccel; Allez, Matthieu; Almeida, Larissa Nogueira; Alvisi, Giorgia; Anderson, Graham; Andrä, Immanuel; Annunziato, Francesco; Anselmo, Achille; Bacher, Petra; Baldari, Cosima T.; Bari, Sudipto; Barnaba, Vincenzo; Borger, Jessica G.; Davey, Martin S.; Quinn, Kylie; Von Borstel, Anouk.

In: European Journal of Immunology, Vol. 49, No. 10, 01.10.2019, p. 1457-1973.

Research output: Contribution to journalArticleOtherpeer-review

TY - JOUR

T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

AU - Cossarizza, Andrea

AU - Chang, Hyun Dong

AU - Radbruch, Andreas

AU - Acs, Andreas

AU - Adam, Dieter

AU - Adam-Klages, Sabine

AU - Agace, William W.

AU - Aghaeepour, Nima

AU - Akdis, Mübeccel

AU - Allez, Matthieu

AU - Almeida, Larissa Nogueira

AU - Alvisi, Giorgia

AU - Anderson, Graham

AU - Andrä, Immanuel

AU - Annunziato, Francesco

AU - Anselmo, Achille

AU - Bacher, Petra

AU - Baldari, Cosima T.

AU - Bari, Sudipto

AU - Barnaba, Vincenzo

AU - Borger, Jessica G.

AU - Davey, Martin S.

AU - Quinn, Kylie

AU - Von Borstel, Anouk

PY - 2019/10/1

Y1 - 2019/10/1

N2 - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

AB - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

U2 - 10.1002/eji.201970107

DO - 10.1002/eji.201970107

M3 - Article

C2 - 31633216

VL - 49

SP - 1457

EP - 1973

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -