Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1457-1973 |
| Number of pages | 517 |
| Journal | European Journal of Immunology |
| Volume | 49 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1 Oct 2019 |
Cite this
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). / Cossarizza, Andrea; Chang, Hyun Dong; Radbruch, Andreas; Acs, Andreas; Adam, Dieter; Adam-Klages, Sabine; Agace, William W.; Aghaeepour, Nima; Akdis, Mübeccel; Allez, Matthieu; Almeida, Larissa Nogueira; Alvisi, Giorgia; Anderson, Graham; Andrä, Immanuel; Annunziato, Francesco; Anselmo, Achille; Bacher, Petra; Baldari, Cosima T.; Bari, Sudipto; Barnaba, Vincenzo; Borger, Jessica G.; Davey, Martin S.; Quinn, Kylie; Von Borstel, Anouk.
In: European Journal of Immunology, Vol. 49, No. 10, 01.10.2019, p. 1457-1973.Research output: Contribution to journal › Article › Other › peer-review
TY - JOUR
T1 - Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
AU - Cossarizza, Andrea
AU - Chang, Hyun Dong
AU - Radbruch, Andreas
AU - Acs, Andreas
AU - Adam, Dieter
AU - Adam-Klages, Sabine
AU - Agace, William W.
AU - Aghaeepour, Nima
AU - Akdis, Mübeccel
AU - Allez, Matthieu
AU - Almeida, Larissa Nogueira
AU - Alvisi, Giorgia
AU - Anderson, Graham
AU - Andrä, Immanuel
AU - Annunziato, Francesco
AU - Anselmo, Achille
AU - Bacher, Petra
AU - Baldari, Cosima T.
AU - Bari, Sudipto
AU - Barnaba, Vincenzo
AU - Borger, Jessica G.
AU - Davey, Martin S.
AU - Quinn, Kylie
AU - Von Borstel, Anouk
PY - 2019/10/1
Y1 - 2019/10/1
N2 - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
AB - These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
U2 - 10.1002/eji.201970107
DO - 10.1002/eji.201970107
M3 - Article
C2 - 31633216
VL - 49
SP - 1457
EP - 1973
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 10
ER -