Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 151 - 175 |
| Number of pages | 24 |
| Journal | Autophagy |
| Volume | 4 |
| Publication status | Published - 2008 |
Cite this
}
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. / Klionsky, Daniel J; Abeliovich, Hagai; Agostinis, Patrizia; Agrawal, Devendra K; Aliev, Gjumrakch; Askew, David S; Baba, Misuzu; Baehrecke, Eric H; Bahr, Ben A; Ballabio, Andrea; Bamber, Bruce A; Bassham, Diane C; Bergamini, Ettore; Bi, Xiaoning; Biard-Piechaczyk, Martine; Blum, Janice S; Bredesen, Dale E; Brodsky, Jeffrey L; Brumell, John H; Brunk, Ulf T; Bursch, Wilfried; Camougrand, Nadine; Cebollero, Eduardo; Cecconi, Francesco; Chen, Yingyu; Chin, Lih-Shen; Choi, Augustine; Chu, Charleen T; Chung, Jongkyeong; Clark, Robert SB; Clarke, Peter GH; Clarke, Steven G; Clave, Corinne; Cleveland, John L; Codogno, Patrice; Colombo, Maria I; Coto-Montes, Aba; Cregg, James M; Cuervo, Ana Maria; Debnath, Jayanta; Dennis, Patrick B; Dennis, Phillip A; Demarchi, Francesca; Deretic, Vojo; Devenish, Rodney James; Di Sano, Frederica; Dice, J Fred; Distelhorst, Clark W; Dinesh-Kumar, Savithramma; Eissa, N Tony; DiFiglia, Marian; Djavaheri-Mergny, Mojgan; Dorsey, Frank C; Droge, Wulf; Dron, Michel; Dunn Jr, William A; Duszenko, Michael; Elazar, Zvulun; Esclatine, Audrey; Eskelinen, Eeva-Liisa; Fesus, Laszlo; Finley, Kim; Fuentes, Jose M; Fueyo, Juan; Fujisaki, Kozo; Galliot, Brigitte; Gao, Fen-Biao; Gewirtz, David A; Gibson, Spencer B; Gohla, Antje; Goldberg, Alfred L; Gonzalez, Ramon; Gonzalez-Estevez, Cristina; Gorski, Sharon; Gottlieb, Roberta A; Haussinger, Dieter; He, You-Wen; Heidenreich, Kim; Hill, Joseph A; Hoyer-Hansen, Maria; Hu, Xun; Huang, Wei-Pang; Iwasaki, Akiko; Jaattela, Marja; Jackson, William T; Jiang, Xuejun; Jin, Shengkan; Johansen, Terje; Jung, Jae U; Kadowaki, Motoni; Kang, Chanhee; Kelekar, Ameeta; Kessel, David H; Kiel, Jan AKW; Kimchi, Adi; Kim, Hong Pyo; Kinsella, Timothy J; Kiselyov, Kirill; Kitamoto, Katsuhiko; Knecht, Erwin; Komatsu, Masaaki; Kominami, Eiki; Kondo, Seiji; Kovacs, Attila L; Kroemer, Guido; Kuan, Chia-Yi; Kumar, Rakesh; Kundu, Mondira; Landry, Jacques; Laporte, Marianne; Le, Weidong; Lei, Huan-Yao; Levine, Beth; Lieberman, Andrew; Lim, Kah-Leong; Lin, Fu-Cheng; Liou, Willisa; Liu, Leroy F; Lopez-Berestein, Gabriel; Lopez-Otin, Carlos; Lu, Bo; MacLeod, Kay F; Malorni, Walter; Martinet, Wim; Matsuoka, Ken; Mautner, Josef; Meijer, Alfred J; Melendez, Alicia; Michels, Paul; Miotto, Giovanni; Mistiaen, Wilhelm P; Mizushima, Noboru; Mogradi, Baharia; Moore, Michael N; Moreira, Paula I; Moriyasu, Yuji; Motyl, Tomasz; Munz, Christian; Murphy, Leon O; Naqvi, Naweed; Neufeld, Thomas P; Nishino, Ichizo; Nixon, Ralph A; Noda, Takeshi; Nurnberg, Bernd; Ogawa, Michinaga; Oleinick, Nancy L; Olsen, Laura J; Ozpolat, Bulent; Paglin, Shoshana; Palmer, Glen E; Papassideri, Issidora; Parkes, Miles; Perlmutter, David H; Perry, George; Piacentini, Mauro; Pinkas-Kramarski, Ronit; Prescott, Mark; Proikas-Cezanne, Tassula; Raben, Nina; Rami, Abdelhaq; Reggiori, Fulvio; Rohrer, Barbel; Rubinsztein, David C; Ryan, Kevin M; Sadoshima, Junichi; Sakagami, Hiroshi; Sakai, Yasuyoshi; Sandri, Marco; Sasakawa, Chihiro; Sass, Miklos; Schneider, Claudio; Seglen, Per O; Seleverstov, Oleksandr; Settleman, Jeffrey; Shacka, John J; Shapiro, Irving M; Sibirny, Andrei; Silva-Zacarin, Elaine CM; Simon, Hans-Uwe; Simone, Cristiano; Simonsen, Anne; Smith, Mark A; Spanel-Borowski, Katharina; Srinivas, Vickram; Steeves, Meredith; Stenmark, Harald; Stromhaug, Per E; Subauste, Carlos S; Sugimoto, Seiichiro; Sulzer, David; Suzuki, Toshihiko; Swanson, Michele S; Tabas, Ira; Takeshita, Fumihiko; Talbot, Nicholas J; Talloczy, Zsolt; Tanaka, Keiji; Tanaka, Kozo; Tanida, Isei; Taylor, Graham S; Taylor, J Paul; Terman, Alexei; Tettamanti, Gianluca; Thompson, Craig B; Thumm, Michael; Tolkovsky, Aviva M; Tooze, Sharon A; Truant, Ray; Tumanovska, Lesya V; Uchiyama, Yasuo; Ueno, Takashi; Uzcategui, Nestor L; van der Klei, Ida; Vaquero, Eva C; Vellai, Tibor; Vogel, Michael W; Wang, Hong-Gang; Webster, Paul; Xi, Zhijun; Xiao, Gutian; Yahalom, Joachim; Yang, Jin-Ming; Yap, George; Yin, Xiao-Ming; Yoshimori, Tamotsu; Yue, Zhenyu; Yuzaki, Michisuke; Zabirnyk, Olga; Zheng, Xiaoxiang; Zhu, Xiongwei; Deter, Russell L.
In: Autophagy, Vol. 4, 2008, p. 151 - 175.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
AU - Klionsky, Daniel J
AU - Abeliovich, Hagai
AU - Agostinis, Patrizia
AU - Agrawal, Devendra K
AU - Aliev, Gjumrakch
AU - Askew, David S
AU - Baba, Misuzu
AU - Baehrecke, Eric H
AU - Bahr, Ben A
AU - Ballabio, Andrea
AU - Bamber, Bruce A
AU - Bassham, Diane C
AU - Bergamini, Ettore
AU - Bi, Xiaoning
AU - Biard-Piechaczyk, Martine
AU - Blum, Janice S
AU - Bredesen, Dale E
AU - Brodsky, Jeffrey L
AU - Brumell, John H
AU - Brunk, Ulf T
AU - Bursch, Wilfried
AU - Camougrand, Nadine
AU - Cebollero, Eduardo
AU - Cecconi, Francesco
AU - Chen, Yingyu
AU - Chin, Lih-Shen
AU - Choi, Augustine
AU - Chu, Charleen T
AU - Chung, Jongkyeong
AU - Clark, Robert SB
AU - Clarke, Peter GH
AU - Clarke, Steven G
AU - Clave, Corinne
AU - Cleveland, John L
AU - Codogno, Patrice
AU - Colombo, Maria I
AU - Coto-Montes, Aba
AU - Cregg, James M
AU - Cuervo, Ana Maria
AU - Debnath, Jayanta
AU - Dennis, Patrick B
AU - Dennis, Phillip A
AU - Demarchi, Francesca
AU - Deretic, Vojo
AU - Devenish, Rodney James
AU - Di Sano, Frederica
AU - Dice, J Fred
AU - Distelhorst, Clark W
AU - Dinesh-Kumar, Savithramma
AU - Eissa, N Tony
AU - DiFiglia, Marian
AU - Djavaheri-Mergny, Mojgan
AU - Dorsey, Frank C
AU - Droge, Wulf
AU - Dron, Michel
AU - Dunn Jr, William A
AU - Duszenko, Michael
AU - Elazar, Zvulun
AU - Esclatine, Audrey
AU - Eskelinen, Eeva-Liisa
AU - Fesus, Laszlo
AU - Finley, Kim
AU - Fuentes, Jose M
AU - Fueyo, Juan
AU - Fujisaki, Kozo
AU - Galliot, Brigitte
AU - Gao, Fen-Biao
AU - Gewirtz, David A
AU - Gibson, Spencer B
AU - Gohla, Antje
AU - Goldberg, Alfred L
AU - Gonzalez, Ramon
AU - Gonzalez-Estevez, Cristina
AU - Gorski, Sharon
AU - Gottlieb, Roberta A
AU - Haussinger, Dieter
AU - He, You-Wen
AU - Heidenreich, Kim
AU - Hill, Joseph A
AU - Hoyer-Hansen, Maria
AU - Hu, Xun
AU - Huang, Wei-Pang
AU - Iwasaki, Akiko
AU - Jaattela, Marja
AU - Jackson, William T
AU - Jiang, Xuejun
AU - Jin, Shengkan
AU - Johansen, Terje
AU - Jung, Jae U
AU - Kadowaki, Motoni
AU - Kang, Chanhee
AU - Kelekar, Ameeta
AU - Kessel, David H
AU - Kiel, Jan AKW
AU - Kimchi, Adi
AU - Kim, Hong Pyo
AU - Kinsella, Timothy J
AU - Kiselyov, Kirill
AU - Kitamoto, Katsuhiko
AU - Knecht, Erwin
AU - Komatsu, Masaaki
AU - Kominami, Eiki
AU - Kondo, Seiji
AU - Kovacs, Attila L
AU - Kroemer, Guido
AU - Kuan, Chia-Yi
AU - Kumar, Rakesh
AU - Kundu, Mondira
AU - Landry, Jacques
AU - Laporte, Marianne
AU - Le, Weidong
AU - Lei, Huan-Yao
AU - Levine, Beth
AU - Lieberman, Andrew
AU - Lim, Kah-Leong
AU - Lin, Fu-Cheng
AU - Liou, Willisa
AU - Liu, Leroy F
AU - Lopez-Berestein, Gabriel
AU - Lopez-Otin, Carlos
AU - Lu, Bo
AU - MacLeod, Kay F
AU - Malorni, Walter
AU - Martinet, Wim
AU - Matsuoka, Ken
AU - Mautner, Josef
AU - Meijer, Alfred J
AU - Melendez, Alicia
AU - Michels, Paul
AU - Miotto, Giovanni
AU - Mistiaen, Wilhelm P
AU - Mizushima, Noboru
AU - Mogradi, Baharia
AU - Moore, Michael N
AU - Moreira, Paula I
AU - Moriyasu, Yuji
AU - Motyl, Tomasz
AU - Munz, Christian
AU - Murphy, Leon O
AU - Naqvi, Naweed
AU - Neufeld, Thomas P
AU - Nishino, Ichizo
AU - Nixon, Ralph A
AU - Noda, Takeshi
AU - Nurnberg, Bernd
AU - Ogawa, Michinaga
AU - Oleinick, Nancy L
AU - Olsen, Laura J
AU - Ozpolat, Bulent
AU - Paglin, Shoshana
AU - Palmer, Glen E
AU - Papassideri, Issidora
AU - Parkes, Miles
AU - Perlmutter, David H
AU - Perry, George
AU - Piacentini, Mauro
AU - Pinkas-Kramarski, Ronit
AU - Prescott, Mark
AU - Proikas-Cezanne, Tassula
AU - Raben, Nina
AU - Rami, Abdelhaq
AU - Reggiori, Fulvio
AU - Rohrer, Barbel
AU - Rubinsztein, David C
AU - Ryan, Kevin M
AU - Sadoshima, Junichi
AU - Sakagami, Hiroshi
AU - Sakai, Yasuyoshi
AU - Sandri, Marco
AU - Sasakawa, Chihiro
AU - Sass, Miklos
AU - Schneider, Claudio
AU - Seglen, Per O
AU - Seleverstov, Oleksandr
AU - Settleman, Jeffrey
AU - Shacka, John J
AU - Shapiro, Irving M
AU - Sibirny, Andrei
AU - Silva-Zacarin, Elaine CM
AU - Simon, Hans-Uwe
AU - Simone, Cristiano
AU - Simonsen, Anne
AU - Smith, Mark A
AU - Spanel-Borowski, Katharina
AU - Srinivas, Vickram
AU - Steeves, Meredith
AU - Stenmark, Harald
AU - Stromhaug, Per E
AU - Subauste, Carlos S
AU - Sugimoto, Seiichiro
AU - Sulzer, David
AU - Suzuki, Toshihiko
AU - Swanson, Michele S
AU - Tabas, Ira
AU - Takeshita, Fumihiko
AU - Talbot, Nicholas J
AU - Talloczy, Zsolt
AU - Tanaka, Keiji
AU - Tanaka, Kozo
AU - Tanida, Isei
AU - Taylor, Graham S
AU - Taylor, J Paul
AU - Terman, Alexei
AU - Tettamanti, Gianluca
AU - Thompson, Craig B
AU - Thumm, Michael
AU - Tolkovsky, Aviva M
AU - Tooze, Sharon A
AU - Truant, Ray
AU - Tumanovska, Lesya V
AU - Uchiyama, Yasuo
AU - Ueno, Takashi
AU - Uzcategui, Nestor L
AU - van der Klei, Ida
AU - Vaquero, Eva C
AU - Vellai, Tibor
AU - Vogel, Michael W
AU - Wang, Hong-Gang
AU - Webster, Paul
AU - Xi, Zhijun
AU - Xiao, Gutian
AU - Yahalom, Joachim
AU - Yang, Jin-Ming
AU - Yap, George
AU - Yin, Xiao-Ming
AU - Yoshimori, Tamotsu
AU - Yue, Zhenyu
AU - Yuzaki, Michisuke
AU - Zabirnyk, Olga
AU - Zheng, Xiaoxiang
AU - Zhu, Xiongwei
AU - Deter, Russell L
PY - 2008
Y1 - 2008
N2 - Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
AB - Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18188003
M3 - Article
VL - 4
SP - 151
EP - 175
JO - Autophagy
JF - Autophagy
SN - 1554-8627
ER -