Guidelines and best practices in successfully using Zebrabow for lineage tracing multiple cells within tissues

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Abstract

Labelling cells and following their progeny, also known as lineage tracing, has provided important insights into the cellular origins of tissues. Traditional lineage tracing experiments have been limited to following single or small groups of cells with classic techniques such as dye injections and Cre/LoxP labelling of cells of interest. Brainbow is a fluorescent dependent, lineage tracing technique that allows a broader visualization and analysis of multiple cells within a tissue, initially deployed to examine lineages within neural tissues. This technique has now been adapted to zebrafish (Zebrabow) and takes advantages of the imaging capabilities that this system provides over other animal models. In this paper we shall describe how Zebrabow is performed as well as some guides on some of the common pitfalls encountered when using this labelling strategy.

Original languageEnglish
Pages (from-to)63-67
Number of pages5
JournalMethods
Volume150
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • Brainbow
  • Imaging
  • Lineage tracing
  • Zebrabow
  • Zebrafish

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