Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme

Gavin J. Knott, Alexandra East-Seletsky, Joshua C. Cofsky, James M. Holton, Emeric Charles, Mitchell R. O'Connell, Jennifer A. Doudna

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42 Citations (Scopus)

Abstract

CRISPR adaptive immune systems protect bacteria from infections by deploying CRISPR RNA (crRNA)-guided enzymes to recognize and cut foreign nucleic acids. Type VI-A CRISPR-Cas systems include the Cas13a enzyme, an RNA-activated RNase capable of crRNA processing and single-stranded RNA degradation upon target-transcript binding. Here we present the 2.0-resolution crystal structure of a crRNA-bound Lachnospiraceae bacterium Cas13a (LbaCas13a), representing a recently discovered Cas13a enzyme subtype. This structure and accompanying biochemical experiments define the Cas13a catalytic residues that are directly responsible for crRNA maturation. In addition, the orientation of the foreign-derived target-RNAspecifying sequence in the protein interior explains the conformational gating of Cas13a nuclease activation. These results describe how Cas13a enzymes generate functional crRNAs and how catalytic activity is blocked before target-RNA recognition, with implications for both bacterial immunity and diagnostic applications.

Original languageEnglish
Pages (from-to)825-833
Number of pages9
JournalNature Structural & Molecular Biology
Volume24
Issue number10
DOIs
Publication statusPublished - 5 Oct 2017
Externally publishedYes

Keywords

  • RNA
  • RNA-binding proteins
  • x-ray crystallography

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