GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women

J. M. Wentworth, Gaetano Naselli, K. Ngui, Gordon K Smyth, R. Liu, P. E. O'Brien, C. Bruce, J. Weir, Michelle Cinel, P. J. Meikle, L C Harrison

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aims:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.Methods:Subcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, lipid and gene profiling were performed.Results:In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in G M3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, phosphatidylethanolamine methyl transferase (PEMT), decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.Interpretation:The relevance of these findings to insulin resistance in humans is supported by published mouse studies, in which adipocyte G M3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-α, impaired insulin action and PEMT was required for adipocyte lipid storage. Thus in visceral adipose tissue of obese humans, an increase in G M3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.

Original languageEnglish
Pages (from-to)706-713
Number of pages8
JournalInternational Journal of Obesity
Volume40
Issue number4
DOIs
Publication statusPublished - 1 Apr 2016

Cite this

Wentworth, J. M. ; Naselli, Gaetano ; Ngui, K. ; Smyth, Gordon K ; Liu, R. ; O'Brien, P. E. ; Bruce, C. ; Weir, J. ; Cinel, Michelle ; Meikle, P. J. ; Harrison, L C. / GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women. In: International Journal of Obesity. 2016 ; Vol. 40, No. 4. pp. 706-713.
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abstract = "Aims:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.Methods:Subcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, lipid and gene profiling were performed.Results:In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in G M3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, phosphatidylethanolamine methyl transferase (PEMT), decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.Interpretation:The relevance of these findings to insulin resistance in humans is supported by published mouse studies, in which adipocyte G M3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-α, impaired insulin action and PEMT was required for adipocyte lipid storage. Thus in visceral adipose tissue of obese humans, an increase in G M3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.",
author = "Wentworth, {J. M.} and Gaetano Naselli and K. Ngui and Smyth, {Gordon K} and R. Liu and O'Brien, {P. E.} and C. Bruce and J. Weir and Michelle Cinel and Meikle, {P. J.} and Harrison, {L C}",
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GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women. / Wentworth, J. M.; Naselli, Gaetano; Ngui, K.; Smyth, Gordon K; Liu, R.; O'Brien, P. E.; Bruce, C.; Weir, J.; Cinel, Michelle; Meikle, P. J.; Harrison, L C.

In: International Journal of Obesity, Vol. 40, No. 4, 01.04.2016, p. 706-713.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - GM3 ganglioside and phosphatidylethanolamine-containing lipids are adipose tissue markers of insulin resistance in obese women

AU - Wentworth, J. M.

AU - Naselli, Gaetano

AU - Ngui, K.

AU - Smyth, Gordon K

AU - Liu, R.

AU - O'Brien, P. E.

AU - Bruce, C.

AU - Weir, J.

AU - Cinel, Michelle

AU - Meikle, P. J.

AU - Harrison, L C

PY - 2016/4/1

Y1 - 2016/4/1

N2 - Aims:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.Methods:Subcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, lipid and gene profiling were performed.Results:In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in G M3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, phosphatidylethanolamine methyl transferase (PEMT), decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.Interpretation:The relevance of these findings to insulin resistance in humans is supported by published mouse studies, in which adipocyte G M3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-α, impaired insulin action and PEMT was required for adipocyte lipid storage. Thus in visceral adipose tissue of obese humans, an increase in G M3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.

AB - Aims:The association between central obesity and insulin resistance reflects the properties of visceral adipose tissue. Our aim was to gain further insight into this association by analysing the lipid composition of subcutaneous and omental adipose tissue in obese women with and without insulin resistance.Methods:Subcutaneous and omental adipose tissue and serum were obtained from 29 obese non-diabetic women, 13 of whom were hyperinsulinemic. Histology, lipid and gene profiling were performed.Results:In omental adipose tissue of obese, insulin-resistant women, adipocyte hypertrophy and macrophage infiltration were accompanied by an increase in G M3 ganglioside and its synthesis enzyme ST3GAL5; in addition, phosphatidylethanolamine (PE) lipids were increased and their degradation enzyme, phosphatidylethanolamine methyl transferase (PEMT), decreased. ST3GAL5 was expressed predominantly in adipose stromovascular cells and PEMT in adipocytes. Insulin resistance was also associated with an increase in PE lipids in serum.Interpretation:The relevance of these findings to insulin resistance in humans is supported by published mouse studies, in which adipocyte G M3 ganglioside, increased by the inflammatory cytokine tumour necrosis factor-α, impaired insulin action and PEMT was required for adipocyte lipid storage. Thus in visceral adipose tissue of obese humans, an increase in G M3 ganglioside secondary to inflammation may contribute to insulin resistance and a decrease in PEMT may be a compensatory response to adipocyte hypertrophy.

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SN - 0307-0565

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