Green chemistry approach for the synthesis of ZnO nanopowders and their cytotoxic effects

Majid Darroudi, Zahra Sabouri, Reza Kazemi Oskuee, Ali Khorsand Zak, Hadi Kargar, Mohamad Hasnul Naim Abd Hamid

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101 Citations (Scopus)


The use of natural polymers in the synthesis of nanomaterials can have a low cost and eco-friendly approach. In this work we would like to report the "green chemistry" synthesis of ZnO nanopowders (ZnO-NPs) using gelatin. Spherical ZnO-NPs were synthesized at different calcination temperatures and field emission scanning electron microscopy (FESEM) imaging showed the formation of most of nanopowders in nanoscale. The powder X-ray diffraction (PXRD) analysis revealed wurtzite hexagonal ZnO with preferential orientation at (101) reflection plane. In vitro cytotoxicity studies on neuro2A cells, a dose dependent toxicity with non-toxic effect of concentration below ~2 μg/mL was shown. The synthesis of ZnO-NPs in gelatinous media were found to be comparable to those obtained from the conventional reduction methods using hazardous polymers or surfactants which can be an excellent alternative for the synthesis of ZnO-NPs using biomaterials.

Original languageEnglish
Pages (from-to)4827-4831
Number of pages5
JournalCeramics International
Issue number3
Publication statusPublished - Apr 2014
Externally publishedYes


  • A. Sol-gel processes
  • B. Electron microscopy
  • D: ZnO

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