GRB10 imprinting is eutherian mammal specific

Jessica Stringer, Shunsuke Suzuki, Andrew Pask, Geoff Shaw, Marilyn Renfree

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

GRB10 is an imprinted gene differently expressed from two promoters in mouse and human. Mouse Grb10 is maternally expressed from the major promoter in most tissues and paternally expressed from the brain-specific promoter within specific regions of the fetal and adult central nervous system. Human GRB10 is biallelically expressed from the major promoter in most tissues except in the placental villus trophoblasts where it is maternally expressed, while the brain-specific promoter is paternally expressed in the fetal brain. This study characterised the orthologue of GRB10 in a marsupial, the tammar wallaby (Macropus eugenii) to investigate the origin and evolution of imprinting at this locus. The protein coding exons and predicted amino acid sequence of tammar GRB10 was highly conserved with eutherian GRB10. The putative first exon, which is located in the orthologous region to the eutherian major promoter, was found in the tammar but no exon was found in the downstream region corresponding to the eutherian brain-specific promoter, suggesting that marsupials only have a single promoter. Tammar GRB10 was widely expressed in various tissues including the brain, but was not imprinted in any of the tissues examined. Thus, it is likely that GRB10 imprinting evolved in eutherians after the eutherian-marsupial divergence approximately 160 million years ago, subsequent to the acquisition of a brain-specific promoter which resides within the imprinting control region in eutherians.
Original languageEnglish
Pages (from-to)3711 - 3719
Number of pages9
JournalMolecular Biology and Evolution
Volume29
Issue number12
DOIs
Publication statusPublished - 2012
Externally publishedYes

Cite this

Stringer, Jessica ; Suzuki, Shunsuke ; Pask, Andrew ; Shaw, Geoff ; Renfree, Marilyn. / GRB10 imprinting is eutherian mammal specific. In: Molecular Biology and Evolution. 2012 ; Vol. 29, No. 12. pp. 3711 - 3719.
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abstract = "GRB10 is an imprinted gene differently expressed from two promoters in mouse and human. Mouse Grb10 is maternally expressed from the major promoter in most tissues and paternally expressed from the brain-specific promoter within specific regions of the fetal and adult central nervous system. Human GRB10 is biallelically expressed from the major promoter in most tissues except in the placental villus trophoblasts where it is maternally expressed, while the brain-specific promoter is paternally expressed in the fetal brain. This study characterised the orthologue of GRB10 in a marsupial, the tammar wallaby (Macropus eugenii) to investigate the origin and evolution of imprinting at this locus. The protein coding exons and predicted amino acid sequence of tammar GRB10 was highly conserved with eutherian GRB10. The putative first exon, which is located in the orthologous region to the eutherian major promoter, was found in the tammar but no exon was found in the downstream region corresponding to the eutherian brain-specific promoter, suggesting that marsupials only have a single promoter. Tammar GRB10 was widely expressed in various tissues including the brain, but was not imprinted in any of the tissues examined. Thus, it is likely that GRB10 imprinting evolved in eutherians after the eutherian-marsupial divergence approximately 160 million years ago, subsequent to the acquisition of a brain-specific promoter which resides within the imprinting control region in eutherians.",
author = "Jessica Stringer and Shunsuke Suzuki and Andrew Pask and Geoff Shaw and Marilyn Renfree",
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Stringer, J, Suzuki, S, Pask, A, Shaw, G & Renfree, M 2012, 'GRB10 imprinting is eutherian mammal specific', Molecular Biology and Evolution, vol. 29, no. 12, pp. 3711 - 3719. https://doi.org/10.1093/molbev/mss173

GRB10 imprinting is eutherian mammal specific. / Stringer, Jessica; Suzuki, Shunsuke; Pask, Andrew; Shaw, Geoff; Renfree, Marilyn.

In: Molecular Biology and Evolution, Vol. 29, No. 12, 2012, p. 3711 - 3719.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - GRB10 imprinting is eutherian mammal specific

AU - Stringer, Jessica

AU - Suzuki, Shunsuke

AU - Pask, Andrew

AU - Shaw, Geoff

AU - Renfree, Marilyn

PY - 2012

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N2 - GRB10 is an imprinted gene differently expressed from two promoters in mouse and human. Mouse Grb10 is maternally expressed from the major promoter in most tissues and paternally expressed from the brain-specific promoter within specific regions of the fetal and adult central nervous system. Human GRB10 is biallelically expressed from the major promoter in most tissues except in the placental villus trophoblasts where it is maternally expressed, while the brain-specific promoter is paternally expressed in the fetal brain. This study characterised the orthologue of GRB10 in a marsupial, the tammar wallaby (Macropus eugenii) to investigate the origin and evolution of imprinting at this locus. The protein coding exons and predicted amino acid sequence of tammar GRB10 was highly conserved with eutherian GRB10. The putative first exon, which is located in the orthologous region to the eutherian major promoter, was found in the tammar but no exon was found in the downstream region corresponding to the eutherian brain-specific promoter, suggesting that marsupials only have a single promoter. Tammar GRB10 was widely expressed in various tissues including the brain, but was not imprinted in any of the tissues examined. Thus, it is likely that GRB10 imprinting evolved in eutherians after the eutherian-marsupial divergence approximately 160 million years ago, subsequent to the acquisition of a brain-specific promoter which resides within the imprinting control region in eutherians.

AB - GRB10 is an imprinted gene differently expressed from two promoters in mouse and human. Mouse Grb10 is maternally expressed from the major promoter in most tissues and paternally expressed from the brain-specific promoter within specific regions of the fetal and adult central nervous system. Human GRB10 is biallelically expressed from the major promoter in most tissues except in the placental villus trophoblasts where it is maternally expressed, while the brain-specific promoter is paternally expressed in the fetal brain. This study characterised the orthologue of GRB10 in a marsupial, the tammar wallaby (Macropus eugenii) to investigate the origin and evolution of imprinting at this locus. The protein coding exons and predicted amino acid sequence of tammar GRB10 was highly conserved with eutherian GRB10. The putative first exon, which is located in the orthologous region to the eutherian major promoter, was found in the tammar but no exon was found in the downstream region corresponding to the eutherian brain-specific promoter, suggesting that marsupials only have a single promoter. Tammar GRB10 was widely expressed in various tissues including the brain, but was not imprinted in any of the tissues examined. Thus, it is likely that GRB10 imprinting evolved in eutherians after the eutherian-marsupial divergence approximately 160 million years ago, subsequent to the acquisition of a brain-specific promoter which resides within the imprinting control region in eutherians.

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SN - 0737-4038

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