Graphene functionalized scaffolds reduce the inflammatory response and supports endogenous neuroblast migration when implanted in the Adult Brain

Kun Zhou, Sepideh Motamed, George A. Thouas, Claude C. Bernard, Dan Li, Helena C. Parkington, Harold A. Coleman, David I. Finkelstein, John S. Forsythe

Research output: Contribution to journalArticleResearchpeer-review

53 Citations (Scopus)

Abstract

Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.

Original languageEnglish
Article numbere0151589
Number of pages15
JournalPLoS ONE
Volume11
Issue number3
DOIs
Publication statusPublished - 15 Mar 2016

Keywords

  • astrocytes
  • microglial cells
  • inflammation
  • microstructure
  • neostriatum
  • neurobiology of disease and regeneration
  • neuronal differentiation
  • brain damage

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