Graphene functionalized scaffolds reduce the inflammatory response and supports endogenous neuroblast migration when implanted in the Adult Brain

Kun Zhou, Sepideh Motamed, George A. Thouas, Claude C. Bernard, Dan Li, Helena C. Parkington, Harold A. Coleman, David I. Finkelstein, John S. Forsythe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.

Original languageEnglish
Article numbere0151589
Number of pages15
JournalPLoS ONE
Volume11
Issue number3
DOIs
Publication statusPublished - 15 Mar 2016

Keywords

  • astrocytes
  • microglial cells
  • inflammation
  • microstructure
  • neostriatum
  • neurobiology of disease and regeneration
  • neuronal differentiation
  • brain damage

Cite this

@article{50587d07bfd749b1af90af274fd33f99,
title = "Graphene functionalized scaffolds reduce the inflammatory response and supports endogenous neuroblast migration when implanted in the Adult Brain",
abstract = "Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.",
keywords = "astrocytes, microglial cells, inflammation, microstructure, neostriatum, neurobiology of disease and regeneration, neuronal differentiation, brain damage",
author = "Kun Zhou and Sepideh Motamed and Thouas, {George A.} and Bernard, {Claude C.} and Dan Li and Parkington, {Helena C.} and Coleman, {Harold A.} and Finkelstein, {David I.} and Forsythe, {John S.}",
year = "2016",
month = "3",
day = "15",
doi = "10.1371/journal.pone.0151589",
language = "English",
volume = "11",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

Graphene functionalized scaffolds reduce the inflammatory response and supports endogenous neuroblast migration when implanted in the Adult Brain. / Zhou, Kun; Motamed, Sepideh; Thouas, George A.; Bernard, Claude C.; Li, Dan; Parkington, Helena C.; Coleman, Harold A.; Finkelstein, David I.; Forsythe, John S.

In: PLoS ONE, Vol. 11, No. 3, e0151589, 15.03.2016.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Graphene functionalized scaffolds reduce the inflammatory response and supports endogenous neuroblast migration when implanted in the Adult Brain

AU - Zhou, Kun

AU - Motamed, Sepideh

AU - Thouas, George A.

AU - Bernard, Claude C.

AU - Li, Dan

AU - Parkington, Helena C.

AU - Coleman, Harold A.

AU - Finkelstein, David I.

AU - Forsythe, John S.

PY - 2016/3/15

Y1 - 2016/3/15

N2 - Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.

AB - Electroactive materials have been investigated as next-generation neuronal tissue engineering scaffolds to enhance neuronal regeneration and functional recovery after brain injury. Graphene, an emerging neuronal scaffold material with charge transfer properties, has shown promising results for neuronal cell survival and differentiation in vitro. In this in vivo work, electrospun microfiber scaffolds coated with self-assembled colloidal graphene, were implanted into the striatum or into the subventricular zone of adult rats. Microglia and astrocyte activation levels were suppressed with graphene functionalization. In addition, self-assembled graphene implants prevented glial scarring in the brain 7 weeks following implantation. Astrocyte guidance within the scaffold and redirection of neuroblasts from the subventricular zone along the implants was also demonstrated. These findings provide new functional evidence for the potential use of graphene scaffolds as a therapeutic platform to support central nervous system regeneration.

KW - astrocytes

KW - microglial cells

KW - inflammation

KW - microstructure

KW - neostriatum

KW - neurobiology of disease and regeneration

KW - neuronal differentiation

KW - brain damage

UR - http://www.scopus.com/inward/record.url?scp=84961603814&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0151589

DO - 10.1371/journal.pone.0151589

M3 - Article

VL - 11

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

M1 - e0151589

ER -