Abstract
Granzyme (grz) AB-/- H2b mice generate numerically normal cytotoxic T lymphocyte (CTL) responses to the prominent influenza A virus Db NP366 and Db PA224 epitopes and terminate the infectious process in the pneumonic lung with the same kinetics as the WT controls. Though grz B protein expression is fully compromised, there is only a partial effect on the level of CTL activity measured in a classical, short-term 51Cr release assay. Single-cell polymerase chain reaction (PCR) analysis of both highly activated effector and "resting" memory CD8+ T cells from influenza A virus-infected grzAB-/- mice showed a high prevalence of grzK mRNA+ expression in tetramer (tet)+ CTLs as was found in WT mice. However, a marked reduction in cytotoxicity present in the primary splenic CTLs of grzAB-/- mice correlated with decreased grzK expression, as measured by real-time PCR. This suggests that grzK plays an important role in CD8+ T-cell cytotoxicity both in the presence and absence of grzA and B.
Original language | English |
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Pages (from-to) | 341-346 |
Number of pages | 6 |
Journal | Viral Immunology |
Volume | 21 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1 Sep 2008 |
Externally published | Yes |