Granulocyte colony-stimulating factor receptor signalling via Janus kinase 2/signal transducer and activator of transcription 3 in ovarian cancer

J. Kumar, F.W. Fraser, C. Riley, N. Ahmed, D.R. McCulloch, A.C. Ward

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

Background:Ovarian cancer remains a major cause of cancer mortality in women, with only limited understanding of disease aetiology at the molecular level. Granulocyte colony-stimulating factor (G-CSF) is a key regulator of both normal and emergency haematopoiesis, and is used clinically to aid haematopoietic recovery following ablative therapies for a variety of solid tumours including ovarian cancer.Methods:The expression of G-CSF and its receptor, G-CSFR, was examined in primary ovarian cancer samples and a panel of ovarian cancer cell lines, and the effects of G-CSF treatment on proliferation, migration and survival were determined.Results:G-CSFR was predominantly expressed in high-grade serous ovarian epithelial tumour samples and a subset of ovarian cancer cell lines. Stimulation of G-CSFR-expressing ovarian epithelial cancer cells with G-CSF led to increased migration and survival, including against chemotherapy-induced apoptosis. The effects of G-CSF were mediated by signalling via the downstream JAK2/STAT3 pathway.Conclusion:This study suggests that G-CSF has the potential to impact on ovarian cancer pathogenesis, and that G-CSFR expression status should be considered in determining appropriate therapy.

Original languageEnglish
Pages (from-to)133-145
Number of pages13
JournalBritish Journal of Cancer
Volume110
Issue number1
DOIs
Publication statusPublished - 7 Jan 2014
Externally publishedYes

Keywords

  • cytokine
  • cytokine receptor
  • G-CSF
  • G-CSFR
  • JAK/STAT
  • ovarian cancer
  • STAT3

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