GPIb-selective activation of platelets induces platelet signaling events comparable to GPVI activation events

Elizabeth Ellen Gardiner, Jane Frances Arthur, Yang Shen, Denuja Karunakaran, Lynley A Moore, Jan Schulte Am Esch II, Robert Keith Andrews, Michael Claude Berndt

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35 Citations (Scopus)

Abstract

We examined platelet aggregation and signaling following selective engagement of platelet GPIb (the major ligand-binding subunit of GPIb-IX-V) by a multivalent surface-expressed GPIb -binding VWF-A1 domain on COS-7 cells. COS-7 cells expressing the VWF-A1 domain containing an R543W mutation (a gain-of-function mutation found in Type 2B von Willebrandi??s Disease) were used as a selective agonist for GPIb-IX-V. When incubated in a cell-to-platelet ratio of up to 1 : 1200, VWF-A1/R543W cells caused rapid, spontaneous aggregation of washed platelets that was GPIb- and IIb3-dependent (blocked by inhibitory anti-VWF-A1, anti-GPIb and anti-IIb3 antibodies).
Original languageEnglish
Pages (from-to)244 - 252
Number of pages9
JournalPlatelets
Volume21
Issue number4
DOIs
Publication statusPublished - 2010

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