TY - JOUR
T1 - GPIb-selective activation of platelets induces platelet signaling events comparable to GPVI activation events
AU - Gardiner, Elizabeth Ellen
AU - Arthur, Jane Frances
AU - Shen, Yang
AU - Karunakaran, Denuja
AU - Moore, Lynley A
AU - Am Esch II, Jan Schulte
AU - Andrews, Robert Keith
AU - Berndt, Michael Claude
PY - 2010
Y1 - 2010
N2 - We examined platelet aggregation and signaling following selective
engagement of platelet GPIb (the major ligand-binding subunit of GPIb-IX-V) by a multivalent surface-expressed
GPIb -binding VWF-A1 domain on COS-7 cells. COS-7 cells expressing the VWF-A1 domain containing an R543W
mutation (a gain-of-function mutation found in Type 2B von Willebrandi??s Disease) were used as a selective agonist for
GPIb-IX-V. When incubated in a cell-to-platelet ratio of up to 1 : 1200, VWF-A1/R543W cells caused rapid, spontaneous aggregation of washed platelets that was GPIb- and IIb3-dependent (blocked by inhibitory anti-VWF-A1, anti-GPIb
and anti-IIb3 antibodies).
AB - We examined platelet aggregation and signaling following selective
engagement of platelet GPIb (the major ligand-binding subunit of GPIb-IX-V) by a multivalent surface-expressed
GPIb -binding VWF-A1 domain on COS-7 cells. COS-7 cells expressing the VWF-A1 domain containing an R543W
mutation (a gain-of-function mutation found in Type 2B von Willebrandi??s Disease) were used as a selective agonist for
GPIb-IX-V. When incubated in a cell-to-platelet ratio of up to 1 : 1200, VWF-A1/R543W cells caused rapid, spontaneous aggregation of washed platelets that was GPIb- and IIb3-dependent (blocked by inhibitory anti-VWF-A1, anti-GPIb
and anti-IIb3 antibodies).
UR - http://informahealthcare.com/doi/pdf/10.3109/09537101003695339
U2 - 10.3109/09537101003695339
DO - 10.3109/09537101003695339
M3 - Article
SN - 0953-7104
VL - 21
SP - 244
EP - 252
JO - Platelets
JF - Platelets
IS - 4
ER -