TY - JOUR
T1 - Gonadal steroids and anterior lobe dynorphin in the male rat
AU - Fullerton, Meryl J.
AU - Smith, A. Ian
AU - Clements, Judith A.
AU - Funder, John W.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Immunoreactive (ir)-dynorphin levels were measured, and the species characterized by high performance liquid chromatography (HPLC), in the pituitary and hypothalamus of intact and castrate male rats. On HPLC, ir-dynorphin co-eluted with authentic dynorphin A 1-8, dynorphin A 1-17 and dynorphin 1-32 in the hypothalamus and intermediate lobe; in two different reversed phase (RP)-HPLC systems, anterior lobe ir-dynorphin co-eluted uniquely with dynorphin 32 (4K dynorphin). Anterior lobe levels of total ir-dynorphin were significantly lowered 7 days after castration, while HPLC profiles in all tissues remained unchanged. The change in anterior pituitary ir-dynorphin levels was reversed in a dose-related manner by dihydrotestosterone (15-500 μg/100 g b. wt/day); estradiol benzoate (3 μg/100 g/day) was without effect. The changes on castration and androgen administration suggest that gonadal steroids play a role in the regulation of dynorphin, as well as gonadotrophins and prolactin, within the anterior pituitary gland.
AB - Immunoreactive (ir)-dynorphin levels were measured, and the species characterized by high performance liquid chromatography (HPLC), in the pituitary and hypothalamus of intact and castrate male rats. On HPLC, ir-dynorphin co-eluted with authentic dynorphin A 1-8, dynorphin A 1-17 and dynorphin 1-32 in the hypothalamus and intermediate lobe; in two different reversed phase (RP)-HPLC systems, anterior lobe ir-dynorphin co-eluted uniquely with dynorphin 32 (4K dynorphin). Anterior lobe levels of total ir-dynorphin were significantly lowered 7 days after castration, while HPLC profiles in all tissues remained unchanged. The change in anterior pituitary ir-dynorphin levels was reversed in a dose-related manner by dihydrotestosterone (15-500 μg/100 g b. wt/day); estradiol benzoate (3 μg/100 g/day) was without effect. The changes on castration and androgen administration suggest that gonadal steroids play a role in the regulation of dynorphin, as well as gonadotrophins and prolactin, within the anterior pituitary gland.
UR - http://www.scopus.com/inward/record.url?scp=0024592348&partnerID=8YFLogxK
U2 - 10.1016/0022-4731(89)90268-9
DO - 10.1016/0022-4731(89)90268-9
M3 - Article
C2 - 2564056
AN - SCOPUS:0024592348
SN - 0022-4731
VL - 32
SP - 303
EP - 308
JO - Journal of Steroid Biochemistry
JF - Journal of Steroid Biochemistry
IS - 2
ER -