Gonadal sex differentiation in Alligator mississippiensis, a species with temperature-dependent sex determination

Craig A. Smith, Jean M.P. Joss

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)

Abstract

Temperature of egg incubation determines sex in Alligator mississippiensis hatchlings. To define the timing and morphology of sexual differentiation, alligator gonads were examined histologically and ultrastructurally throughout embryogenesis. At the male-producing temperature (33° C), the onset of testis differentiation occurred in most embryos during developmental stages 21-22, when a number of somatic cells in the medulla of the gonad became enlarged, forming presumptive Sertoli cells. Some enlarged somatic cells were also observed at the female-producing temperature (30° C) during gonadogenesis, but they were less widespread than at 33° C. Ovarian differentiation at 30° C began slighlty later, during stage 22-23, and was characterised by proliferation of germs cells in the cortex of the gonad. Testis formation in alligators may depend upon presumptive Sertoli cells differentiating prior to a critical event in embryogenesis, such as germ cell proliferation and meiosis. If follows that ovary formation occurs if this requirement is not met, as at lower incubation temperatures.

Original languageEnglish
Pages (from-to)149-162
Number of pages14
JournalCell and Tissue Research
Volume273
Issue number1
DOIs
Publication statusPublished - 1 Jul 1993
Externally publishedYes

Keywords

  • Alligator mississippiensis (Crocodilia)
  • Gonadal differentiation
  • Ovary
  • Sertoli cells
  • Temperature-dependent sex determination
  • Testis

Cite this

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abstract = "Temperature of egg incubation determines sex in Alligator mississippiensis hatchlings. To define the timing and morphology of sexual differentiation, alligator gonads were examined histologically and ultrastructurally throughout embryogenesis. At the male-producing temperature (33° C), the onset of testis differentiation occurred in most embryos during developmental stages 21-22, when a number of somatic cells in the medulla of the gonad became enlarged, forming presumptive Sertoli cells. Some enlarged somatic cells were also observed at the female-producing temperature (30° C) during gonadogenesis, but they were less widespread than at 33° C. Ovarian differentiation at 30° C began slighlty later, during stage 22-23, and was characterised by proliferation of germs cells in the cortex of the gonad. Testis formation in alligators may depend upon presumptive Sertoli cells differentiating prior to a critical event in embryogenesis, such as germ cell proliferation and meiosis. If follows that ovary formation occurs if this requirement is not met, as at lower incubation temperatures.",
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Gonadal sex differentiation in Alligator mississippiensis, a species with temperature-dependent sex determination. / Smith, Craig A.; Joss, Jean M.P.

In: Cell and Tissue Research, Vol. 273, No. 1, 01.07.1993, p. 149-162.

Research output: Contribution to journalArticleResearchpeer-review

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