Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation

Jessica M Olsen, Masaaki Sato, Olof S Dallner, Anna L Sandstrom, Didier F Pisani, Jean-Claude Chambard, Ez-Zoubir Amri, Dana S Hutchinson, Tore Bengtsson

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Abstract

Brown adipose tissue is the primary site for thermogenesis and can consume, in addition to free fatty acids, a very high amount of glucose from the blood, which can both acutely and chronically affect glucose homeostasis. Here, we show that mechanistic target of rapamycin (mTOR) complex 2 has a novel role in beta3-adrenoceptor-stimulated glucose uptake in brown adipose tissue. We show that beta3-adrenoceptors stimulate glucose uptake in brown adipose tissue via a signaling pathway that is comprised of two different parts: one part dependent on cAMP-mediated increases in GLUT1 transcription and de novo synthesis of GLUT1 and another part dependent on mTOR complex 2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane, leading to increased glucose uptake. Both parts are essential for beta3-adrenoceptor-stimulated glucose uptake. Importantly, the effect of beta3-adrenoceptor on mTOR complex 2 is independent of the classical insulin-phosphoinositide 3-kinase-Akt pathway, highlighting a novel mechanism of mTOR complex 2 activation.
Original languageEnglish
Pages (from-to)365 - 374
Number of pages10
JournalJournal of Cell Biology
Volume207
Issue number3
DOIs
Publication statusPublished - 2014

Cite this

Olsen, J. M., Sato, M., Dallner, O. S., Sandstrom, A. L., Pisani, D. F., Chambard, J-C., ... Bengtsson, T. (2014). Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation. Journal of Cell Biology, 207(3), 365 - 374. https://doi.org/10.1083/jcb.201403080
Olsen, Jessica M ; Sato, Masaaki ; Dallner, Olof S ; Sandstrom, Anna L ; Pisani, Didier F ; Chambard, Jean-Claude ; Amri, Ez-Zoubir ; Hutchinson, Dana S ; Bengtsson, Tore. / Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation. In: Journal of Cell Biology. 2014 ; Vol. 207, No. 3. pp. 365 - 374.
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abstract = "Brown adipose tissue is the primary site for thermogenesis and can consume, in addition to free fatty acids, a very high amount of glucose from the blood, which can both acutely and chronically affect glucose homeostasis. Here, we show that mechanistic target of rapamycin (mTOR) complex 2 has a novel role in beta3-adrenoceptor-stimulated glucose uptake in brown adipose tissue. We show that beta3-adrenoceptors stimulate glucose uptake in brown adipose tissue via a signaling pathway that is comprised of two different parts: one part dependent on cAMP-mediated increases in GLUT1 transcription and de novo synthesis of GLUT1 and another part dependent on mTOR complex 2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane, leading to increased glucose uptake. Both parts are essential for beta3-adrenoceptor-stimulated glucose uptake. Importantly, the effect of beta3-adrenoceptor on mTOR complex 2 is independent of the classical insulin-phosphoinositide 3-kinase-Akt pathway, highlighting a novel mechanism of mTOR complex 2 activation.",
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Olsen, JM, Sato, M, Dallner, OS, Sandstrom, AL, Pisani, DF, Chambard, J-C, Amri, E-Z, Hutchinson, DS & Bengtsson, T 2014, 'Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation', Journal of Cell Biology, vol. 207, no. 3, pp. 365 - 374. https://doi.org/10.1083/jcb.201403080

Glucose uptake in brown fat cells is dependent on mTOR complex 2-promoted GLUT1 translocation. / Olsen, Jessica M; Sato, Masaaki; Dallner, Olof S; Sandstrom, Anna L; Pisani, Didier F; Chambard, Jean-Claude; Amri, Ez-Zoubir; Hutchinson, Dana S; Bengtsson, Tore.

In: Journal of Cell Biology, Vol. 207, No. 3, 2014, p. 365 - 374.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Sato, Masaaki

AU - Dallner, Olof S

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AU - Pisani, Didier F

AU - Chambard, Jean-Claude

AU - Amri, Ez-Zoubir

AU - Hutchinson, Dana S

AU - Bengtsson, Tore

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AB - Brown adipose tissue is the primary site for thermogenesis and can consume, in addition to free fatty acids, a very high amount of glucose from the blood, which can both acutely and chronically affect glucose homeostasis. Here, we show that mechanistic target of rapamycin (mTOR) complex 2 has a novel role in beta3-adrenoceptor-stimulated glucose uptake in brown adipose tissue. We show that beta3-adrenoceptors stimulate glucose uptake in brown adipose tissue via a signaling pathway that is comprised of two different parts: one part dependent on cAMP-mediated increases in GLUT1 transcription and de novo synthesis of GLUT1 and another part dependent on mTOR complex 2-stimulated translocation of newly synthesized GLUT1 to the plasma membrane, leading to increased glucose uptake. Both parts are essential for beta3-adrenoceptor-stimulated glucose uptake. Importantly, the effect of beta3-adrenoceptor on mTOR complex 2 is independent of the classical insulin-phosphoinositide 3-kinase-Akt pathway, highlighting a novel mechanism of mTOR complex 2 activation.

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