Global Metabolic Analyses of Acinetobacter baumannii

Mohd Hafidz Mahamad Maifiah, Tony Velkov, Darren J. Creek, Jian Li

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

Abstract

Acinetobacter baumannii is rapidly emerging as a multidrug-resistant pathogen responsible for nosocomial infections including pneumonia, bacteremia, wound infections, urinary tract infections, and meningitis. Metabolomics provides a powerful tool to gain a system-wide snapshot of cellular biochemical networks under defined conditions and has been increasingly applied to bacterial physiology and drug discovery. Here we describe an optimized sample preparation method for untargeted metabolomics studies in A. baumannii. Our method provides a significant recovery of intracellular metabolites to demonstrate substantial differences in global metabolic profiles among A. baumannii strains.

Original languageEnglish
Title of host publicationAcinetobacter baumannii
Subtitle of host publicationMethods and Protocols
EditorsIndranil Biswas, Philip N. Rather
PublisherHumana Press
Pages321-328
Number of pages8
Edition1
ISBN (Electronic)9781493991181
ISBN (Print)9781493991174
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
Volume1946
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Acinetobacter baumannii
  • Metabolomics
  • Multidrug-resistant
  • Polymyxins

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