Global functional knockdown of ATP binding cassette transporter A1 stimulates development of atherosclerosis in apoE K/O mice

Nigora Mukhamedova, Wilissa D'Souza, Hann Low, Rajitha Kesani, Giovanna Chimini, Dmitri Sviridov

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4 Citations (Scopus)

Abstract

ABCA1 is a key element of cellular cholesterol homeostasis. ApoE K/O mice fed with high-fat diet were infused with anti-ABCA1 antibody or control IgM. Infusion of anti-ABCA1 antibody led to 72% increase in the area of atherosclerotic plaque in aorta. After 16. weeks on high-fat diet plasma level of high density lipoprotein cholesterol (HDL-C) was reduced in control group, but was unchanged in mice infused with anti-ABCA1 antibody. Total plasma cholesterol level was elevated while the capacity of plasma to support cholesterol efflux ex vivo was reduced after 16. weeks on high-fat diet; the effects were similar in the two groups. We conclude that functional blocking of ABCA1-dependent cholesterol efflux stimulates development of atherosclerosis in apoE K/O mice independently from HDL-C levels.

Original languageEnglish
Pages (from-to)446-449
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume412
Issue number3
DOIs
Publication statusPublished - 2 Sep 2011
Externally publishedYes

Keywords

  • ABCA1
  • Atherosclerosis
  • HDL

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