Glioma surgical aspirate: A viable source of tumor tissue for experimental research

Bryan Day, Brett W Stringer, John Wilson, Rosalind L Jeffree, Paul R Jamieson, Kathleen S Ensbey, Zara C Bruce, Po L Inglis, Suzanne Allan, Craig Winter, Gert Tollesson, Scott F Campbell, Peter Lucas, Wendy Findlay, David Kadrian, David Johnson, Thomas Robertson, Terrance Grant Johns, Perry Bartlett, Geoffrey W OsborneAndrew W Boyd

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.
Original languageEnglish
Pages (from-to)357 - 371
Number of pages15
JournalCancers
Volume5
Issue number2
DOIs
Publication statusPublished - 2013

Cite this

Day, B., Stringer, B. W., Wilson, J., Jeffree, R. L., Jamieson, P. R., Ensbey, K. S., ... Boyd, A. W. (2013). Glioma surgical aspirate: A viable source of tumor tissue for experimental research. Cancers, 5(2), 357 - 371. https://doi.org/10.3390/cancers5020357
Day, Bryan ; Stringer, Brett W ; Wilson, John ; Jeffree, Rosalind L ; Jamieson, Paul R ; Ensbey, Kathleen S ; Bruce, Zara C ; Inglis, Po L ; Allan, Suzanne ; Winter, Craig ; Tollesson, Gert ; Campbell, Scott F ; Lucas, Peter ; Findlay, Wendy ; Kadrian, David ; Johnson, David ; Robertson, Thomas ; Johns, Terrance Grant ; Bartlett, Perry ; Osborne, Geoffrey W ; Boyd, Andrew W. / Glioma surgical aspirate: A viable source of tumor tissue for experimental research. In: Cancers. 2013 ; Vol. 5, No. 2. pp. 357 - 371.
@article{97591b245e764c0eba21c217efdd3593,
title = "Glioma surgical aspirate: A viable source of tumor tissue for experimental research",
abstract = "Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.",
author = "Bryan Day and Stringer, {Brett W} and John Wilson and Jeffree, {Rosalind L} and Jamieson, {Paul R} and Ensbey, {Kathleen S} and Bruce, {Zara C} and Inglis, {Po L} and Suzanne Allan and Craig Winter and Gert Tollesson and Campbell, {Scott F} and Peter Lucas and Wendy Findlay and David Kadrian and David Johnson and Thomas Robertson and Johns, {Terrance Grant} and Perry Bartlett and Osborne, {Geoffrey W} and Boyd, {Andrew W}",
year = "2013",
doi = "10.3390/cancers5020357",
language = "English",
volume = "5",
pages = "357 -- 371",
journal = "Cancers",
issn = "2072-6694",
publisher = "MDPI",
number = "2",

}

Day, B, Stringer, BW, Wilson, J, Jeffree, RL, Jamieson, PR, Ensbey, KS, Bruce, ZC, Inglis, PL, Allan, S, Winter, C, Tollesson, G, Campbell, SF, Lucas, P, Findlay, W, Kadrian, D, Johnson, D, Robertson, T, Johns, TG, Bartlett, P, Osborne, GW & Boyd, AW 2013, 'Glioma surgical aspirate: A viable source of tumor tissue for experimental research', Cancers, vol. 5, no. 2, pp. 357 - 371. https://doi.org/10.3390/cancers5020357

Glioma surgical aspirate: A viable source of tumor tissue for experimental research. / Day, Bryan; Stringer, Brett W; Wilson, John; Jeffree, Rosalind L; Jamieson, Paul R; Ensbey, Kathleen S; Bruce, Zara C; Inglis, Po L; Allan, Suzanne; Winter, Craig; Tollesson, Gert; Campbell, Scott F; Lucas, Peter; Findlay, Wendy; Kadrian, David; Johnson, David; Robertson, Thomas; Johns, Terrance Grant; Bartlett, Perry; Osborne, Geoffrey W; Boyd, Andrew W.

In: Cancers, Vol. 5, No. 2, 2013, p. 357 - 371.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Glioma surgical aspirate: A viable source of tumor tissue for experimental research

AU - Day, Bryan

AU - Stringer, Brett W

AU - Wilson, John

AU - Jeffree, Rosalind L

AU - Jamieson, Paul R

AU - Ensbey, Kathleen S

AU - Bruce, Zara C

AU - Inglis, Po L

AU - Allan, Suzanne

AU - Winter, Craig

AU - Tollesson, Gert

AU - Campbell, Scott F

AU - Lucas, Peter

AU - Findlay, Wendy

AU - Kadrian, David

AU - Johnson, David

AU - Robertson, Thomas

AU - Johns, Terrance Grant

AU - Bartlett, Perry

AU - Osborne, Geoffrey W

AU - Boyd, Andrew W

PY - 2013

Y1 - 2013

N2 - Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.

AB - Brain cancer research has been hampered by a paucity of viable clinical tissue of sufficient quality and quantity for experimental research. This has driven researchers to rely heavily on long term cultured cells which no longer represent the cancers from which they were derived. Resection of brain tumors, particularly at the interface between normal and tumorigenic tissue, can be carried out using an ultrasonic surgical aspirator (CUSA) that deposits liquid (blood and irrigation fluid) and resected tissue into a sterile bottle for disposal. To determine the utility of CUSA-derived glioma tissue for experimental research, we collected 48 CUSA specimen bottles from glioma patients and analyzed both the solid tissue fragments and dissociated tumor cells suspended in the liquid waste fraction. We investigated if these fractions would be useful for analyzing tumor heterogeneity, using IHC and multi-parameter flow cytometry; we also assessed culture generation and orthotopic xenograft potential. Both cell sources proved to be an abundant, highly viable source of live tumor cells for cytometric analysis, animal studies and in-vitro studies. Our findings demonstrate that CUSA tissue represents an abundant viable source to conduct experimental research and to carry out diagnostic analyses by flow cytometry or other molecular diagnostic procedures.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3730332/pdf/cancers-05-00357.pdf

U2 - 10.3390/cancers5020357

DO - 10.3390/cancers5020357

M3 - Article

VL - 5

SP - 357

EP - 371

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 2

ER -