Giant MACPF/CDC pore forming toxins

A class of their own

Research output: Contribution to journalArticleResearchpeer-review

20 Citations (Scopus)

Abstract

Pore Forming Toxins (PFTs) represent a key mechanism for permitting the passage of proteins and small molecules across the lipid membrane. These proteins are typically produced as soluble monomers that self-assemble into ring-like oligomeric structures on the membrane surface. Following such assembly PFTs undergo a remarkable conformational change to insert into the lipid membrane. While many different protein families have independently evolved such ability, members of the Membrane Attack Complex PerForin/Cholesterol Dependent Cytolysin (MACPF/CDC) superfamily form distinctive giant beta-barrel pores comprised of up to 50 monomers and up to 300A in diameter. In this review we focus on recent advances in understanding the structure of these giant MACPF/CDC pores as well as the underlying molecular mechanisms leading to their formation. Commonalities and evolved variations of the pore forming mechanism across the superfamily are discussed. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale.
Original languageEnglish
Pages (from-to)475-486
Number of pages12
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1858
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016

Keywords

  • MACPF
  • CDC
  • Pore forming toxin
  • Perforin
  • Membrane attack complex
  • Pleurotolysin

Cite this

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title = "Giant MACPF/CDC pore forming toxins: A class of their own",
abstract = "Pore Forming Toxins (PFTs) represent a key mechanism for permitting the passage of proteins and small molecules across the lipid membrane. These proteins are typically produced as soluble monomers that self-assemble into ring-like oligomeric structures on the membrane surface. Following such assembly PFTs undergo a remarkable conformational change to insert into the lipid membrane. While many different protein families have independently evolved such ability, members of the Membrane Attack Complex PerForin/Cholesterol Dependent Cytolysin (MACPF/CDC) superfamily form distinctive giant beta-barrel pores comprised of up to 50 monomers and up to 300A in diameter. In this review we focus on recent advances in understanding the structure of these giant MACPF/CDC pores as well as the underlying molecular mechanisms leading to their formation. Commonalities and evolved variations of the pore forming mechanism across the superfamily are discussed. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale.",
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Giant MACPF/CDC pore forming toxins : A class of their own. / Reboul, Cyril F; Whisstock, James C; Dunstone, Michelle A.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1858, No. 3, 01.03.2016, p. 475-486.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

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T2 - A class of their own

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KW - MACPF

KW - CDC

KW - Pore forming toxin

KW - Perforin

KW - Membrane attack complex

KW - Pleurotolysin

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