Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 280 - 290 |
| Number of pages | 11 |
| Journal | Endocrinology |
| Volume | 156 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2015 |
Cite this
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Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). / Ku, Jacqueline; Andrews, Zane B; Barsby, Tom; Reichenbach, Alexander; Lemus, Moyra B; Drummond, Grant R; Sleeman, Mark; Spencer, Sarah J; Sobey, Christopher G; Miller, Alyson A.
In: Endocrinology, Vol. 156, No. 1, 2015, p. 280 - 290.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)
AU - Ku, Jacqueline
AU - Andrews, Zane B
AU - Barsby, Tom
AU - Reichenbach, Alexander
AU - Lemus, Moyra B
AU - Drummond, Grant R
AU - Sleeman, Mark
AU - Spencer, Sarah J
AU - Sobey, Christopher G
AU - Miller, Alyson A
PY - 2015
Y1 - 2015
N2 - The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values
AB - The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272401/
U2 - 10.1210/en.2014-1415
DO - 10.1210/en.2014-1415
M3 - Article
VL - 156
SP - 280
EP - 290
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 1
ER -