Projects per year
Abstract
The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values
Original language | English |
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Pages (from-to) | 280 - 290 |
Number of pages | 11 |
Journal | Endocrinology |
Volume | 156 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2015 |
Projects
- 2 Finished
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NHMRC Research Fellowship
National Health and Medical Research Council (NHMRC) (Australia)
1/01/05 → 31/12/19
Project: Research