Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)

Jacqueline Ku; Zane B Andrews; Tom Barsby; Alexander Reichenbach; Moyra B Lemus; Grant R Drummond; Mark Sleeman; Sarah J Spencer; Christopher G Sobey; Alyson A Miller

doi:10.1210/en.2014-1415

Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)

Jacqueline Ku, Zane B Andrews, Tom Barsby, Alexander Reichenbach, Moyra B Lemus, Grant R Drummond, Mark Sleeman, Sarah J Spencer, Christopher G Sobey, Alyson A Miller

Research output: Contribution to journal › Article › Research › peer-review

21 Citations (Scopus)

Abstract

The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values

Original language	English
Pages (from-to)	280 - 290
Number of pages	11
Journal	Endocrinology
Volume	156
Issue number	1
DOIs	https://doi.org/10.1210/en.2014-1415
Publication status	Published - 2015

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Ku, J., Andrews, Z. B., Barsby, T., Reichenbach, A., Lemus, M. B., Drummond, G. R., Sleeman, M., Spencer, S. J., Sobey, C. G., & Miller, A. A. (2015). Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). Endocrinology, 156(1), 280 - 290. https://doi.org/10.1210/en.2014-1415

Ku, Jacqueline ; Andrews, Zane B ; Barsby, Tom ; Reichenbach, Alexander ; Lemus, Moyra B ; Drummond, Grant R ; Sleeman, Mark ; Spencer, Sarah J ; Sobey, Christopher G ; Miller, Alyson A. / Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). In: Endocrinology. 2015 ; Vol. 156, No. 1. pp. 280 - 290.

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title = "Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)",

abstract = "The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values",

author = "Jacqueline Ku and Andrews, {Zane B} and Tom Barsby and Alexander Reichenbach and Lemus, {Moyra B} and Drummond, {Grant R} and Mark Sleeman and Spencer, {Sarah J} and Sobey, {Christopher G} and Miller, {Alyson A}",

year = "2015",

doi = "10.1210/en.2014-1415",

language = "English",

volume = "156",

pages = "280 -- 290",

journal = "Endocrinology",

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In: Endocrinology, Vol. 156, No. 1, 2015, p. 280 - 290.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)

AU - Ku, Jacqueline

AU - Andrews, Zane B

AU - Barsby, Tom

AU - Reichenbach, Alexander

AU - Lemus, Moyra B

AU - Drummond, Grant R

AU - Sleeman, Mark

AU - Spencer, Sarah J

AU - Sobey, Christopher G

AU - Miller, Alyson A

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AB - The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values

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