Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s)

Jacqueline Ku, Zane B Andrews, Tom Barsby, Alexander Reichenbach, Moyra B Lemus, Grant R Drummond, Mark Sleeman, Sarah J Spencer, Christopher G Sobey, Alyson A Miller

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values
Original languageEnglish
Pages (from-to)280 - 290
Number of pages11
JournalEndocrinology
Volume156
Issue number1
DOIs
Publication statusPublished - 2015

Cite this

Ku, Jacqueline ; Andrews, Zane B ; Barsby, Tom ; Reichenbach, Alexander ; Lemus, Moyra B ; Drummond, Grant R ; Sleeman, Mark ; Spencer, Sarah J ; Sobey, Christopher G ; Miller, Alyson A. / Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). In: Endocrinology. 2015 ; Vol. 156, No. 1. pp. 280 - 290.
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abstract = "The ghrelin-related peptides, acylated ghrelin, des-acylated ghrelin, and obestatin, are novel gastrointestinal hormones. We firstly investigated whether the ghrelin gene, GOAT, and the ghrelin receptor (growth hormone secretagogue receptor 1a [GHSR1a]) are expressed in mouse cerebral arteries. Secondly, we assessed the cerebrovascular actions of ghrelin-related peptides by examining their effects on vasodilator nitric oxide (NO) and superoxide production. Using RT-PCR, we found the ghrelin gene and GOAT to be expressed at negligible levels in cerebral arteries from male wild-type mice. mRNA expression of GHSR1a was also found to be low in cerebral arteries, and GHSR protein was undetectable in GHSR-eGFP mice. We next found that exogenous acylated ghrelin had no effect on the tone of perfused cerebral arteries, or superoxide production. By contrast, exogenous des-acylated ghrelin or obestatin elicited powerful vasodilator responses (EC50 values",
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Ghrelin-related peptides exert protective effects in the cerebral circulation of male mice through a nonclassical ghrelin receptor(s). / Ku, Jacqueline; Andrews, Zane B; Barsby, Tom; Reichenbach, Alexander; Lemus, Moyra B; Drummond, Grant R; Sleeman, Mark; Spencer, Sarah J; Sobey, Christopher G; Miller, Alyson A.

In: Endocrinology, Vol. 156, No. 1, 2015, p. 280 - 290.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ku, Jacqueline

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