Germline-encoded recognition of diverse glycolipids by natural killer T cells

James P Scott-Browne, Jennifer L Matsuda, Thierry Mallevaey, Janice White, Natalie Borg, James McCluskey, Jamie Rossjohn, John Kappler, Philippa Marrack, Laurent Gapin

Research output: Contribution to journalArticleResearchpeer-review

130 Citations (Scopus)

Abstract

Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.
Original languageEnglish
Pages (from-to)1105 - 1113
Number of pages9
JournalNature Immunology
Volume8
Issue number10
Publication statusPublished - 2007

Cite this

Scott-Browne, J. P., Matsuda, J. L., Mallevaey, T., White, J., Borg, N., McCluskey, J., ... Gapin, L. (2007). Germline-encoded recognition of diverse glycolipids by natural killer T cells. Nature Immunology, 8(10), 1105 - 1113.
Scott-Browne, James P ; Matsuda, Jennifer L ; Mallevaey, Thierry ; White, Janice ; Borg, Natalie ; McCluskey, James ; Rossjohn, Jamie ; Kappler, John ; Marrack, Philippa ; Gapin, Laurent. / Germline-encoded recognition of diverse glycolipids by natural killer T cells. In: Nature Immunology. 2007 ; Vol. 8, No. 10. pp. 1105 - 1113.
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abstract = "Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.",
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Scott-Browne, JP, Matsuda, JL, Mallevaey, T, White, J, Borg, N, McCluskey, J, Rossjohn, J, Kappler, J, Marrack, P & Gapin, L 2007, 'Germline-encoded recognition of diverse glycolipids by natural killer T cells', Nature Immunology, vol. 8, no. 10, pp. 1105 - 1113.

Germline-encoded recognition of diverse glycolipids by natural killer T cells. / Scott-Browne, James P; Matsuda, Jennifer L; Mallevaey, Thierry; White, Janice; Borg, Natalie; McCluskey, James; Rossjohn, Jamie; Kappler, John; Marrack, Philippa; Gapin, Laurent.

In: Nature Immunology, Vol. 8, No. 10, 2007, p. 1105 - 1113.

Research output: Contribution to journalArticleResearchpeer-review

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AU - McCluskey, James

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Scott-Browne JP, Matsuda JL, Mallevaey T, White J, Borg N, McCluskey J et al. Germline-encoded recognition of diverse glycolipids by natural killer T cells. Nature Immunology. 2007;8(10):1105 - 1113.