Germline-encoded recognition of diverse glycolipids by natural killer T cells

James P Scott-Browne; Jennifer L Matsuda; Thierry Mallevaey; Janice White; Natalie Borg; James McCluskey; Jamie Rossjohn; John Kappler; Philippa Marrack; Laurent Gapin

Germline-encoded recognition of diverse glycolipids by natural killer T cells

James P Scott-Browne, Jennifer L Matsuda, Thierry Mallevaey, Janice White, Natalie Borg, James McCluskey, Jamie Rossjohn, John Kappler, Philippa Marrack, Laurent Gapin

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › Research › peer-review

139 Citations (Scopus)

Abstract

Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.

Original language	English
Pages (from-to)	1105 - 1113
Number of pages	9
Journal	Nature Immunology
Volume	8
Issue number	10
Publication status	Published - 2007

Cite this

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title = "Germline-encoded recognition of diverse glycolipids by natural killer T cells",

abstract = "Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.",

author = "Scott-Browne, {James P} and Matsuda, {Jennifer L} and Thierry Mallevaey and Janice White and Natalie Borg and James McCluskey and Jamie Rossjohn and John Kappler and Philippa Marrack and Laurent Gapin",

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T1 - Germline-encoded recognition of diverse glycolipids by natural killer T cells

AU - Scott-Browne, James P

AU - Matsuda, Jennifer L

AU - Mallevaey, Thierry

AU - White, Janice

AU - Borg, Natalie

AU - McCluskey, James

AU - Rossjohn, Jamie

AU - Kappler, John

AU - Marrack, Philippa

AU - Gapin, Laurent

PY - 2007

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N2 - Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.

AB - Natural killer T cells expressing invariant T cell receptor alpha-chains (TCRalpha chains) containing variable (V) and joining (J) region V(alpha)14-J(alpha)18 (V(alpha)14i) rearrangements recognize both endogenous and microbial glycolipids in the context of CD1d. How cells expressing an invariant TCRalpha chain and a restricted set of TCRbeta chains recognize structurally diverse antigens is not clear. Here we show that a V(alpha)14i TCR recognized many alpha-linked glycolipids by means of a hot-spot of germline-encoded amino acids in complementarity-determining regions 3alpha, 1alpha and 2beta. This hot-spot did not shift during the recognition of structurally distinct antigens, suggesting that the V(alpha)14i TCR functions as a pattern-recognition receptor, conferring on natural killer T cells the ability to sense and respond in an innate way to pathogens displaying antigenic alpha-linked glycolipids.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17828267

M3 - Article

SN - 1529-2908

VL - 8

SP - 1105

EP - 1113

JO - Nature Immunology

JF - Nature Immunology

IS - 10

ER -

Germline-encoded recognition of diverse glycolipids by natural killer T cells

Abstract

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