Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8 + T cell response

Abigail Culshaw, Kristin Ladell, Stephanie Gras, James E. McLaren, Kelly L. Miners, Carine Farenc, Heleen Van Den Heuvel, Emma Gostick, Wanwisa Dejnirattisai, Apirath Wangteeraprasert, Thaneeya Duangchinda, Pojchong Chotiyarnwong, Wannee Limpitikul, Sirijitt Vasanawathana, Prida Malasit, Tao Dong, Jamie Rossjohn, Juthathip Mongkolsapaya, David A. Price, Gavin R. Screaton

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Adaptive immune responses protect against infection with dengue virus (DENV), yet cross-reactivity with distinct serotypes can precipitate life-threatening clinical disease. We found that clonotypes expressing the T cell antigen receptor (TCR) β-chain variable region 11 (TRBV11-2) were 'preferentially' activated and mobilized within immunodominant human-leukocyte-antigen-(HLA)-A∗11:01-restricted CD8 + T cell populations specific for variants of the nonstructural protein epitope NS3 133 that characterize the serotypes DENV1, DENV3 and DENV4. In contrast, the NS3 133 -DENV2-specific repertoire was largely devoid of such TCRs. Structural analysis of a representative TRBV11-2 + TCR demonstrated that cross-serotype reactivity was governed by unique interplay between the variable antigenic determinant and germline-encoded residues in the second β-chain complementarity-determining region (CDR2β). Extensive mutagenesis studies of three distinct TRBV11-2 + TCRs further confirmed that antigen recognition was dependent on key contacts between the serotype-defined peptide and discrete residues in the CDR2β loop. Collectively, these data reveal an innate-like mode of epitope recognition with potential implications for the outcome of sequential exposure to heterologous DENVs.

Original languageEnglish
Pages (from-to)1228-1237
Number of pages10
JournalNature Immunology
Volume18
Issue number11
DOIs
Publication statusPublished - 18 Oct 2017

Keywords

  • immunology
  • infection

Cite this

Culshaw, Abigail ; Ladell, Kristin ; Gras, Stephanie ; McLaren, James E. ; Miners, Kelly L. ; Farenc, Carine ; Van Den Heuvel, Heleen ; Gostick, Emma ; Dejnirattisai, Wanwisa ; Wangteeraprasert, Apirath ; Duangchinda, Thaneeya ; Chotiyarnwong, Pojchong ; Limpitikul, Wannee ; Vasanawathana, Sirijitt ; Malasit, Prida ; Dong, Tao ; Rossjohn, Jamie ; Mongkolsapaya, Juthathip ; Price, David A. ; Screaton, Gavin R. / Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8 + T cell response. In: Nature Immunology. 2017 ; Vol. 18, No. 11. pp. 1228-1237.
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abstract = "Adaptive immune responses protect against infection with dengue virus (DENV), yet cross-reactivity with distinct serotypes can precipitate life-threatening clinical disease. We found that clonotypes expressing the T cell antigen receptor (TCR) β-chain variable region 11 (TRBV11-2) were 'preferentially' activated and mobilized within immunodominant human-leukocyte-antigen-(HLA)-A∗11:01-restricted CD8 + T cell populations specific for variants of the nonstructural protein epitope NS3 133 that characterize the serotypes DENV1, DENV3 and DENV4. In contrast, the NS3 133 -DENV2-specific repertoire was largely devoid of such TCRs. Structural analysis of a representative TRBV11-2 + TCR demonstrated that cross-serotype reactivity was governed by unique interplay between the variable antigenic determinant and germline-encoded residues in the second β-chain complementarity-determining region (CDR2β). Extensive mutagenesis studies of three distinct TRBV11-2 + TCRs further confirmed that antigen recognition was dependent on key contacts between the serotype-defined peptide and discrete residues in the CDR2β loop. Collectively, these data reveal an innate-like mode of epitope recognition with potential implications for the outcome of sequential exposure to heterologous DENVs.",
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author = "Abigail Culshaw and Kristin Ladell and Stephanie Gras and McLaren, {James E.} and Miners, {Kelly L.} and Carine Farenc and {Van Den Heuvel}, Heleen and Emma Gostick and Wanwisa Dejnirattisai and Apirath Wangteeraprasert and Thaneeya Duangchinda and Pojchong Chotiyarnwong and Wannee Limpitikul and Sirijitt Vasanawathana and Prida Malasit and Tao Dong and Jamie Rossjohn and Juthathip Mongkolsapaya and Price, {David A.} and Screaton, {Gavin R.}",
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Culshaw, A, Ladell, K, Gras, S, McLaren, JE, Miners, KL, Farenc, C, Van Den Heuvel, H, Gostick, E, Dejnirattisai, W, Wangteeraprasert, A, Duangchinda, T, Chotiyarnwong, P, Limpitikul, W, Vasanawathana, S, Malasit, P, Dong, T, Rossjohn, J, Mongkolsapaya, J, Price, DA & Screaton, GR 2017, 'Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8 + T cell response' Nature Immunology, vol. 18, no. 11, pp. 1228-1237. https://doi.org/10.1038/ni.3850

Germline bias dictates cross-serotype reactivity in a common dengue-virus-specific CD8 + T cell response. / Culshaw, Abigail; Ladell, Kristin; Gras, Stephanie; McLaren, James E.; Miners, Kelly L.; Farenc, Carine; Van Den Heuvel, Heleen; Gostick, Emma; Dejnirattisai, Wanwisa; Wangteeraprasert, Apirath; Duangchinda, Thaneeya; Chotiyarnwong, Pojchong; Limpitikul, Wannee; Vasanawathana, Sirijitt; Malasit, Prida; Dong, Tao; Rossjohn, Jamie; Mongkolsapaya, Juthathip; Price, David A.; Screaton, Gavin R.

In: Nature Immunology, Vol. 18, No. 11, 18.10.2017, p. 1228-1237.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Culshaw, Abigail

AU - Ladell, Kristin

AU - Gras, Stephanie

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AU - Miners, Kelly L.

AU - Farenc, Carine

AU - Van Den Heuvel, Heleen

AU - Gostick, Emma

AU - Dejnirattisai, Wanwisa

AU - Wangteeraprasert, Apirath

AU - Duangchinda, Thaneeya

AU - Chotiyarnwong, Pojchong

AU - Limpitikul, Wannee

AU - Vasanawathana, Sirijitt

AU - Malasit, Prida

AU - Dong, Tao

AU - Rossjohn, Jamie

AU - Mongkolsapaya, Juthathip

AU - Price, David A.

AU - Screaton, Gavin R.

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N2 - Adaptive immune responses protect against infection with dengue virus (DENV), yet cross-reactivity with distinct serotypes can precipitate life-threatening clinical disease. We found that clonotypes expressing the T cell antigen receptor (TCR) β-chain variable region 11 (TRBV11-2) were 'preferentially' activated and mobilized within immunodominant human-leukocyte-antigen-(HLA)-A∗11:01-restricted CD8 + T cell populations specific for variants of the nonstructural protein epitope NS3 133 that characterize the serotypes DENV1, DENV3 and DENV4. In contrast, the NS3 133 -DENV2-specific repertoire was largely devoid of such TCRs. Structural analysis of a representative TRBV11-2 + TCR demonstrated that cross-serotype reactivity was governed by unique interplay between the variable antigenic determinant and germline-encoded residues in the second β-chain complementarity-determining region (CDR2β). Extensive mutagenesis studies of three distinct TRBV11-2 + TCRs further confirmed that antigen recognition was dependent on key contacts between the serotype-defined peptide and discrete residues in the CDR2β loop. Collectively, these data reveal an innate-like mode of epitope recognition with potential implications for the outcome of sequential exposure to heterologous DENVs.

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