TY - JOUR
T1 - Genotyping, morphology and molecular characteristics of a lytic phage of Neisseria strain obtained from infected human dental plaque
AU - Aljarbou, Ahmed N
AU - Aljofan, Mohamad
PY - 2014
Y1 - 2014
N2 - The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60+/-3 nm), a sheathed and rigid long tail ( approximately 175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24+/-2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.
AB - The lytic bacteriaphage (phage) A2 was isolated from human dental plaques along with its bacterial host. The virus was found to have an icosahedron-shaped head (60+/-3 nm), a sheathed and rigid long tail ( approximately 175 nm) and was categorized into the family Siphoviridae of the order Caudovirales, which are dsDNA viral family, characterised by their ability to infect bacteria and are nonenveloped with a noncontractile tail. The isolated phage contained a linear dsDNA genome having 31,703 base pairs of unique sequence, which were sorted into three contigs and 12 single sequences. A latent period of 25 minutes and burst size of 24+/-2 particles was determined for the virus. Bioinformatics approaches were used to identify ORFs in the genome. A phylogenetic analysis confirmed the species inter-relationship and its placement in the family.
UR - http://link.springer.com/article/10.1007%2Fs12275-014-3380-1
U2 - 10.1007/s12275-014-3380-1
DO - 10.1007/s12275-014-3380-1
M3 - Article
VL - 52
SP - 609
EP - 618
JO - The Journal of Microbiology
JF - The Journal of Microbiology
SN - 1225-8873
IS - 7
ER -