TY - JOUR
T1 - Genotypic and metabolic approaches towards the segregation of Klebsiella pneumoniae strains producing different antibiotic resistant enzymes
AU - Low, Ye Mun
AU - Yap, Ivan Kok Seng
AU - Abdul Jabar, Kartini
AU - Md Yusof, Mohd Yasim
AU - Chong, Chun Wie
AU - Teh, Cindy Shuan Ju
N1 - Funding Information:
This work was supported by Fundamental Research Grant Scheme (FRGS) from the Ministry of Education, Malaysia (Grant Number: FP023-2014A), University of Malaya Research Grant (UMRG) from University of Malaya (Grant Number: RP014B-14HTM) and Postgraduate Research Grant (PPP) from University of Malaya (Grant Number: PG028-2014B).
Publisher Copyright:
© 2017, Springer Science+Business Media New York.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/5
Y1 - 2017/5
N2 - Introduction: Genotype and metabolomic variation are important for bacterial survival and adaptation to environmental changes. Objectives: In this study, we compared the relationship among Klebsiella pneumoniae strains based on their genotypic and metabolic profiles. In addition, we also evaluated the association of the relationship with beta-lactamase production. Methods: A total of 53 K. pneumoniae strains isolated in 2013–2014 from a tertiary teaching hospital in Malaysia were subjected to antimicrobial susceptibility testing (AST) via disk diffusion method and beta-lactamase production confirmation. The bacterial strains were also typed genotypically and metabolically via REP-PCR and 1H-NMR spectroscopy respectively. The concordance of the matrices derived based on genotypic and metabolic characterization was measured based on Spearman’s rank correlation. Results: Spearman’s correlation rank showed that there is a weak but significant negative correlation between the genetic fingerprints and metabolic profiles of K. pneumoniae. Specifically, K. pneumoniae strains were clustered into five major clusters based on REP-PCR where most of the carbapenem resistant K. pneumoniae (CRKP) strains made up the major cluster. In contrast, metabolic patterns of the three groups (i.e. CRKP, extended spectrum beta-lactamase producing K. pneumoniae (ESBL), susceptible) of K. pneumoniae were clearly differentiated on PLS-DA score plots derived from 1H-NMR spectroscopy. Conclusion: Overall, this study showed that metabolomic profiling using 1H-NMR spectroscopy is able to discriminate K. pneumoniae strains based on their beta-lactamase production status.
AB - Introduction: Genotype and metabolomic variation are important for bacterial survival and adaptation to environmental changes. Objectives: In this study, we compared the relationship among Klebsiella pneumoniae strains based on their genotypic and metabolic profiles. In addition, we also evaluated the association of the relationship with beta-lactamase production. Methods: A total of 53 K. pneumoniae strains isolated in 2013–2014 from a tertiary teaching hospital in Malaysia were subjected to antimicrobial susceptibility testing (AST) via disk diffusion method and beta-lactamase production confirmation. The bacterial strains were also typed genotypically and metabolically via REP-PCR and 1H-NMR spectroscopy respectively. The concordance of the matrices derived based on genotypic and metabolic characterization was measured based on Spearman’s rank correlation. Results: Spearman’s correlation rank showed that there is a weak but significant negative correlation between the genetic fingerprints and metabolic profiles of K. pneumoniae. Specifically, K. pneumoniae strains were clustered into five major clusters based on REP-PCR where most of the carbapenem resistant K. pneumoniae (CRKP) strains made up the major cluster. In contrast, metabolic patterns of the three groups (i.e. CRKP, extended spectrum beta-lactamase producing K. pneumoniae (ESBL), susceptible) of K. pneumoniae were clearly differentiated on PLS-DA score plots derived from 1H-NMR spectroscopy. Conclusion: Overall, this study showed that metabolomic profiling using 1H-NMR spectroscopy is able to discriminate K. pneumoniae strains based on their beta-lactamase production status.
KW - Carbapenem resistant
KW - Enterobacteriaceae
KW - Extended spectrum beta-lactamase (ESBL)
KW - Metabolic profile
KW - Nuclear magnetic resonance (NMR)
KW - Repetitive extragenic palindromic PCR (REP-PCR)
UR - http://www.scopus.com/inward/record.url?scp=85017141863&partnerID=8YFLogxK
U2 - 10.1007/s11306-017-1201-3
DO - 10.1007/s11306-017-1201-3
M3 - Article
AN - SCOPUS:85017141863
VL - 13
JO - Metabolomics
JF - Metabolomics
SN - 1573-3882
IS - 5
M1 - 65
ER -