GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER

M. A. Degli‐Esposti; J. F. Williamson; G. K. Tay; B. Salmon; G. A. Joseph; A. A. Morley; R. L. Dawkins

doi:10.1111/j.1744-313X.1993.tb00156.x

GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER

M. A. Degli‐Esposti, J. F. Williamson, G. K. Tay, B. Salmon, G. A. Joseph, A. A. Morley, R. L. Dawkins

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti‐HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.

Original language	English
Pages (from-to)	373-380
Number of pages	8
Journal	International Journal of Immunogenetics
Volume	20
Issue number	5
DOIs	https://doi.org/10.1111/j.1744-313X.1993.tb00156.x
Publication status	Published - 1 Jan 1993
Externally published	Yes

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title = "GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER",

abstract = "The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti‐HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.",

author = "Degli‐Esposti, {M. A.} and Williamson, {J. F.} and Tay, {G. K.} and B. Salmon and Joseph, {G. A.} and Morley, {A. A.} and Dawkins, {R. L.}",

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AU - Degli‐Esposti, M. A.

AU - Williamson, J. F.

AU - Tay, G. K.

AU - Salmon, B.

AU - Joseph, G. A.

AU - Morley, A. A.

AU - Dawkins, R. L.

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Y1 - 1993/1/1

N2 - The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti‐HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.

AB - The aim of this study was to characterize MHC mutant cell lines by studying haplospecific markers within the MHC and specifically in the 250 kilobase (kb) region between the HLA B and TNF loci. This region has been difficult to define because of the lack of appropriate markers. Spontaneous MHC mutants were isolated after immunoselection with an anti‐HLA A2 monoclonal antibody and complement. Ten mutants were characterized using serological or allelic and genomic DNA markers within the HLA A to HLA DQ region of the MHC. Most mutants lost at least the 3 megabases of DNA from HLA A to HLA DQ viz the whole haplotype carrying HLA A2. Variants which have lost either HLA A alone or HLA A and HLA B were also found. The results show that it is possible to map the extent of the deletion between HLA B and TNF. Haplospecific scanning patterns for the CL region appear particularly useful.

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GENOMIC MHC HAPLOTYPING OF MUTANT CELL LINES USING A NOVEL MARKER

Abstract

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