Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Mashkoor Mohsin; Mariya Azam; Sajjad Ur Sajjad-Ur-Rahman; Fernanda Esposito; Fábio P. Sellera; Daniel F. Monte; Louise Cerdeira; Nilton Lincopan

doi:10.1093/femspd/ftz064

Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Mashkoor Mohsin, Mariya Azam, Sajjad Ur Sajjad-Ur-Rahman, Fernanda Esposito, Fábio P. Sellera, Daniel F. Monte, Louise Cerdeira, Nilton Lincopan

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

Original language	English
Article number	ftz064
Number of pages	4
Journal	Pathogens and Disease
Volume	77
Issue number	7
DOIs	https://doi.org/10.1093/femspd/ftz064
Publication status	Published - 1 Oct 2019
Externally published	Yes

Keywords

Galleria mellonella
poultry
resistome; colistin resistance
virulome
WGS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/femspd/ftz064

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title = "Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan",

abstract = "The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4\% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.",

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author = "Mashkoor Mohsin and Mariya Azam and Sajjad-Ur-Rahman, \{Sajjad Ur\} and Fernanda Esposito and Sellera, \{F{\'a}bio P.\} and Monte, \{Daniel F.\} and Louise Cerdeira and Nilton Lincopan",

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AU - Mohsin, Mashkoor

AU - Azam, Mariya

AU - Sajjad-Ur-Rahman, Sajjad Ur

AU - Esposito, Fernanda

AU - Sellera, Fábio P.

AU - Monte, Daniel F.

AU - Cerdeira, Louise

AU - Lincopan, Nilton

PY - 2019/10/1

Y1 - 2019/10/1

N2 - The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

AB - The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

KW - Galleria mellonella

KW - poultry

KW - resistome; colistin resistance

KW - virulome

KW - WGS

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DO - 10.1093/femspd/ftz064

M3 - Article

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SN - 2049-632X

VL - 77

JO - Pathogens and Disease

JF - Pathogens and Disease

IS - 7

M1 - ftz064

ER -

Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Abstract

Keywords

UN SDGs

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