Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Mashkoor Mohsin; Mariya Azam; Sajjad Ur Sajjad-Ur-Rahman; Fernanda Esposito; Fábio P. Sellera; Daniel F. Monte; Louise Cerdeira; Nilton Lincopan

doi:10.1093/femspd/ftz064

Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Mashkoor Mohsin, Mariya Azam, Sajjad Ur Sajjad-Ur-Rahman, Fernanda Esposito, Fábio P. Sellera, Daniel F. Monte, Louise Cerdeira, Nilton Lincopan

Research output: Contribution to journal › Article › Research › peer-review

11 Citations (Scopus)

Abstract

The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

Original language	English
Article number	ftz064
Number of pages	4
Journal	Pathogens and Disease
Volume	77
Issue number	7
DOIs	https://doi.org/10.1093/femspd/ftz064
Publication status	Published - 1 Oct 2019
Externally published	Yes

Keywords

Galleria mellonella
poultry
resistome; colistin resistance
virulome
WGS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/femspd/ftz064

Cite this

@article{8c02c9a6239f4f999f251b49e97c3bc2,

title = "Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan",

abstract = "The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.",

keywords = "Galleria mellonella, poultry, resistome; colistin resistance, virulome, WGS",

author = "Mashkoor Mohsin and Mariya Azam and Sajjad-Ur-Rahman, {Sajjad Ur} and Fernanda Esposito and Sellera, {F{\'a}bio P.} and Monte, {Daniel F.} and Louise Cerdeira and Nilton Lincopan",

year = "2019",

month = oct,

day = "1",

doi = "10.1093/femspd/ftz064",

language = "English",

volume = "77",

journal = "Pathogens and Disease",

issn = "2049-632X",

publisher = "Oxford University Press",

number = "7",

}

TY - JOUR

T1 - Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

AU - Mohsin, Mashkoor

AU - Azam, Mariya

AU - Sajjad-Ur-Rahman, Sajjad Ur

AU - Esposito, Fernanda

AU - Sellera, Fábio P.

AU - Monte, Daniel F.

AU - Cerdeira, Louise

AU - Lincopan, Nilton

PY - 2019/10/1

Y1 - 2019/10/1

N2 - The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

AB - The convergence of high virulence and multidrug resistance (MDR) in Gram-negative pathogens circulating at the human-animal interface is a critical public health issue. We hereby report the genomic characteristics and virulent behavior of a colistin-resistant Escherichia coli, serotype ONT:H26, belonging to ST6395, isolated from a healthy broiler in Pakistan. This strain harbored multiple antimicrobial resistance genes, including mcr-1.1 and blaCARB-2, besides cma (colicin M) and astA [heat-stable enterotoxin 1 (EAST1) toxin] virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that MCR-1-positive E. coli ST6395 killed 96.4% of the larvae at 18 hour post-infection. Interplay between resistance and virulence in clinically important pathogens could be a potential threat, representing a serious challenge to global public health.

KW - Galleria mellonella

KW - poultry

KW - resistome; colistin resistance

KW - virulome

KW - WGS

UR - http://www.scopus.com/inward/record.url?scp=85077017178&partnerID=8YFLogxK

U2 - 10.1093/femspd/ftz064

DO - 10.1093/femspd/ftz064

M3 - Article

C2 - 31782775

SN - 2049-632X

VL - 77

JO - Pathogens and Disease

JF - Pathogens and Disease

IS - 7

M1 - ftz064

ER -

Genomic background of a colistin-resistant and highly virulent MCR-1-positive Escherichia coli ST6395 from a broiler chicken in Pakistan

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Cite this