Genome-wide transcription responses to synchrotron microbeam radiotherapy

Carl Norman Sprung, Yuqing Yang, Helen Barbara Forrester, Jason Li, Marina Skrypnik, Leonie Miriam Cann, Christina Restall, Robin Anderson, Jeffrey Crosbie, Peter Adrian Walton Rogers

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18 Citations (Scopus)

Abstract

The majority of cancer patients achieve benefit from radiotherapy. A significant limitation of radiotherapy is its relatively low therapeutic index, defined as the maximum radiation dose that causes acceptable normal tissue damage to the minimum dose required to achieve tumor control. Recently, a new radiotherapy modality using synchrotron-generated X-ray microbeam radiotherapy has been demonstrated in animal models to ablate tumors with concurrent sparing of normal tissue. Very little work has been undertaken into the cellular and molecular mechanisms that differentiate microbeam radiotherapy from broad beam. The purpose of this study was to investigate and compare the whole genome transcriptional response of in vivo microbeam radiotherapy versus broad beam irradiated tumors. We hypothesized that gene expression changes after microbeam radiotherapy are different from those seen after broad beam. We found that in EMT6.5 tumors at 4-48 h postirradiation, microbeam radiotherapy differentially regulates a number of genes, including major histocompatibility complex (MHC) class II antigen gene family members, and other immunity-related genes including Ciita, Ifng, Cxcl1, Cxcl9, Indo and Ubd when compared to broad beam. Our findings demonstrate molecular differences in the tumor response to microbeam versus broad beam irradiation and these differences provide insight into the underlying mechanisms between microbeam radiotherapy and broad beam
Original languageEnglish
Pages (from-to)249 - 259
Number of pages11
JournalRadiation Research
Volume178
Issue number4
DOIs
Publication statusPublished - 2012

Cite this

Sprung, C. N., Yang, Y., Forrester, H. B., Li, J., Skrypnik, M., Cann, L. M., Restall, C., Anderson, R., Crosbie, J., & Rogers, P. A. W. (2012). Genome-wide transcription responses to synchrotron microbeam radiotherapy. Radiation Research, 178(4), 249 - 259. https://doi.org/10.1667/RR2885.1