Genome-wide ENU mutagenesis in combination with high density SNP analysis and exome sequencing provides rapid identification of novel mouse models of developmental disease

Georgina Caruana, Peter Gordon Farlie, Adam Howie Hart, Stefan Bagheri-Fam, Megan Jane Wallace, Michael Serge Dobbie, Christopher T Gordon, Kerry A Miller, Belinda Whittle, Helen Elizabeth Abud, Ruth M Arkell, Timothy James Cole, Vincent Russel Harley, Ian Macleod Smyth, John Frederick Bertram

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BACKGROUND: Mice harbouring gene mutations that cause phenotypic abnormalities during organogenesis are invaluable tools for linking gene function to normal development and human disorders. To generate mouse models harbouring novel alleles that are involved in organogenesis we conducted a phenotype-driven, genome-wide mutagenesis screen in mice using the mutagen -ethyl--nitrosourea (ENU). METHODOLOGYPRINCIPAL FINDINGS: ENU was injected into male C57BL/6 mice and the mutations transmitted through the germ-line. ENU-induced mutations were bred to homozygosity and G3 embryos screened at embryonic day (E) 13.5 and E18.5 for abnormalities in limb and craniofacial structures, skin, blood, vasculature, lungs, gut, kidneys, ureters and gonads. From 52 pedigrees screened 15 were detected with anomalies in one or more of the structures/organs screened. Using single nucleotide polymorphism (SNP)-based linkage analysis in conjunction with candidate gene or next-generation sequencing (NGS) we identified novel recessive alleles for and CONCLUSIONSSIGNIFICANCE: In this study we have generated mouse models in which the anomalies closely mimic those seen in human disorders. The association between novel mutant alleles and phenotypes will lead to a better understanding of gene function in normal development and establish how their dysfunction causes human anomalies and disease.
Original languageEnglish
Article numbere55429
Number of pages14
JournalPLoS ONE
Issue number3
Publication statusPublished - 2013

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