Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

Quézia Moura; Fernanda Esposito; Miriam R. Fernandes; Maria Espinoza-Muñoz; Tiago A. Souza; Silvia R. Santos; Louise Cerdeira; Valéria Cassettari; Nilton Lincopan

doi:10.1093/femspd/ftx121

Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

Quézia Moura
, Fernanda Esposito
, Miriam R. Fernandes
, Maria Espinoza-Muñoz
, Tiago A. Souza
, Silvia R. Santos
, Louise Cerdeira
, Valéria Cassettari
, Nilton Lincopan

Research output: Contribution to journal › Article › Research › peer-review

18 Citations (Scopus)

Abstract

The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.

Original language	English
Journal	Pathogens and Disease
Volume	75
Issue number	9
DOIs	https://doi.org/10.1093/femspd/ftx121
Publication status	Published - 29 Dec 2017
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Brazil
extended-spectrum beta-lactamase
Galleria mellonella
hvKP
K. pneumoniae
whole genome sequence

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10.1093/femspd/ftx121

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title = "Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection",

abstract = "The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100\% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.",

keywords = "Brazil, extended-spectrum beta-lactamase, Galleria mellonella, hvKP, K. pneumoniae, whole genome sequence",

author = "Qu{\'e}zia Moura and Fernanda Esposito and Fernandes, \{Miriam R.\} and Maria Espinoza-Mu{\~n}oz and Souza, \{Tiago A.\} and Santos, \{Silvia R.\} and Louise Cerdeira and Val{\'e}ria Cassettari and Nilton Lincopan",

year = "2017",

month = dec,

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doi = "10.1093/femspd/ftx121",

language = "English",

volume = "75",

journal = "Pathogens and Disease",

issn = "2049-632X",

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T1 - Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

AU - Moura, Quézia

AU - Esposito, Fernanda

AU - Fernandes, Miriam R.

AU - Espinoza-Muñoz, Maria

AU - Souza, Tiago A.

AU - Santos, Silvia R.

AU - Cerdeira, Louise

AU - Cassettari, Valéria

AU - Lincopan, Nilton

PY - 2017/12/29

Y1 - 2017/12/29

N2 - The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.

AB - The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage.

KW - Brazil

KW - extended-spectrum beta-lactamase

KW - Galleria mellonella

KW - hvKP

KW - K. pneumoniae

KW - whole genome sequence

UR - https://www.scopus.com/pages/publications/85050319365

U2 - 10.1093/femspd/ftx121

DO - 10.1093/femspd/ftx121

M3 - Article

C2 - 29228178

AN - SCOPUS:85050319365

SN - 2049-632X

VL - 75

JO - Pathogens and Disease

JF - Pathogens and Disease

IS - 9

ER -

Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

Abstract

UN SDGs

Keywords

Access to Document

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