Genome organization of the Pseudomonas aeruginosa narrow host range plasmid R91–5 determined by deletion and cloning analysis

By physical and genetic analysis of deletion mutants of the narrow host range IncP-10 P. aeruginosa conjugative plasmid R91–5 it has been shown that the phenotypes related to its transfer, namely the inhibition of the replication of the phage G101, entry exclusion and the fertility inhibition of the wide host range plasmid R18 map at kilobase coordinates 19·7–20·7, 18·5–19·7, 28·8–30·15 and/or 34·9–36·15, respectively. These locations have been confirmed by cloning restriction enzyme generated DNA fragments of R91–5 into a small broad host range, multicopy non-conjugative plasmid.