Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs).

Amir Taheri-Ghahfarokhi, Luis F Malaver Ortega, Huseyin Sumer

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

Interest is increasing in transcription activator-like effector nucleases (TALENs) as a tool to introduce targeted double-strand breaks into the large genomes of human and animal cell lines. The produced DNA lesions stimulate DNA repair pathways, error-prone but dominant non-homologous end joining (NHEJ) and accurate but less occurring homology-directed repair (HDR), and as a result targeted genes can be modifi ed. Here, we describe a modifi ed Golden-Gate cloning method for generating TALENs and also details for targeting genes in mouse embryonic stem cells. The protocol described here can be used for modifying the genome of a broad range of pluripotent cell lines.
Original languageEnglish
Title of host publicationCell Reprogramming
Subtitle of host publicationMethods and Protocols
EditorsPaul J Verma, Huseyin Sumer
Place of PublicationNew York NYJ USA
PublisherHumana Press
Pages253-268
Number of pages16
Edition1st
ISBN (Print)9781493928477
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Number1330
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Cite this

Taheri-Ghahfarokhi, A., Malaver Ortega, L. F., & Sumer, H. (2015). Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs). In P. J. Verma, & H. Sumer (Eds.), Cell Reprogramming: Methods and Protocols (1st ed., pp. 253-268). (Methods in Molecular Biology: Springer Protocols; No. 1330). New York NYJ USA: Humana Press. https://doi.org/10.1007/978-1-4939-2848-4
Taheri-Ghahfarokhi, Amir ; Malaver Ortega, Luis F ; Sumer, Huseyin. / Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs). Cell Reprogramming: Methods and Protocols. editor / Paul J Verma ; Huseyin Sumer. 1st. ed. New York NYJ USA : Humana Press, 2015. pp. 253-268 (Methods in Molecular Biology: Springer Protocols; 1330).
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Taheri-Ghahfarokhi, A, Malaver Ortega, LF & Sumer, H 2015, Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs). in PJ Verma & H Sumer (eds), Cell Reprogramming: Methods and Protocols. 1st edn, Methods in Molecular Biology: Springer Protocols, no. 1330, Humana Press, New York NYJ USA, pp. 253-268. https://doi.org/10.1007/978-1-4939-2848-4

Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs). / Taheri-Ghahfarokhi, Amir; Malaver Ortega, Luis F; Sumer, Huseyin.

Cell Reprogramming: Methods and Protocols. ed. / Paul J Verma; Huseyin Sumer. 1st. ed. New York NYJ USA : Humana Press, 2015. p. 253-268 (Methods in Molecular Biology: Springer Protocols; No. 1330).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Taheri-Ghahfarokhi A, Malaver Ortega LF, Sumer H. Genome Modification of Pluripotent Cells by Using Transcription Activator-Like Effector Nucleases (TALENs). In Verma PJ, Sumer H, editors, Cell Reprogramming: Methods and Protocols. 1st ed. New York NYJ USA: Humana Press. 2015. p. 253-268. (Methods in Molecular Biology: Springer Protocols; 1330). https://doi.org/10.1007/978-1-4939-2848-4