Genetic relatedness among Lansium domesticum accessions using RAPD markers

Beng Kah Song, Mahani Mansor Clyde, Ratnam Wickneswari, Mohd Noor Normah

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Genetic relatedness among 85 Lansium domesticum Corr. accessions from Peninsular Malaysia were investigated using random amplified polymorphic DNA (RAPD) markers. Ten primers were used for amplification and yielded a total of 113 bands, of which 107 were polymorphic. Homology tests showed that the RAPD bands used in the study satisfy assumptions of homology and non-allelic behaviour. A dendrogram showing genetic similarities among accessions was constructed based on the 107 polymorphic bands using UPGMA cluster analysis. Jaccard similarity coefficients ranged from 0.25 to 1.00 among accessions indicating a diverse genepool in the species indicative of different species parentage. The dendrogram separated the 85 accessions into three main clusters, one comprising 56 accessions which possess thin-skinned fruit (mostly Dokong and Langsat), while the second has 28 accessions (mostly Duku-langsat, Duku Terengganu and Duku Johor) with thick fruit skin and the third comprising only one accession, namely Duku hutan. Thus, RAPD analysis was a useful tool for determining the genetic relatedness among accessions and identifying different types of L. domesticum. (C) 2000 Annals of Botany Company.

Original languageEnglish
Pages (from-to)299-307
Number of pages9
JournalAnnals of Botany
Issue number2
Publication statusPublished - 2000
Externally publishedYes


  • Cluster analysis
  • Genetic relatedness
  • Lansium domesticum
  • RAPD markers

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