Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR + T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8+ and CD4+ T lymphocytes for in vitro and in vivo characterization.