Genetic modification of mouse effector and helper T lymphocytes expressing a chimeric antigen receptor

Liza B John, Tess Madison Chee, David E Gilham, Phillip K Darcy

Research output: Contribution to journalArticleOther

2 Citations (Scopus)

Abstract

Genetic modification of primary mouse T cells with chimeric antigen receptors (CAR) has emerged as an important tool for optimizing adoptive T cell immunotherapy strategies. However, limitations in current protocols for generating highly pure and sufficient numbers of enriched effector and helper CAR + T cell subsets remain problematic. Here, we describe a new retroviral transduction protocol for successfully generating transduced CD8+ and CD4+ T lymphocytes for in vitro and in vivo characterization.
Original languageEnglish
Pages (from-to)177 - 187
Number of pages11
JournalOncoImmunology
Volume1139
Publication statusPublished - 2014

Cite this