Genetic instability in EBV-transformed lymphoblastoid cell lines

Aisha Mohyuddin; Qasim Ayub; Saima Siddiqi; Denise R. Carvalho-Silva; Kehkashan Mazhar; Sadia Rehman; Sadaf Firasat; Ahsana Dar; Chris Tyler-Smith; S. Qasim Mehdi

doi:10.1016/j.bbagen.2003.10.014

Genetic instability in EBV-transformed lymphoblastoid cell lines

Aisha Mohyuddin, Qasim Ayub, Saima Siddiqi, Denise R. Carvalho-Silva, Kehkashan Mazhar, Sadia Rehman, Sadaf Firasat, Ahsana Dar, Chris Tyler-Smith, S. Qasim Mehdi

Research output: Contribution to journal › Short Survey › Research › peer-review

29 Citations (Scopus)

Abstract

Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.

Original language	English
Pages (from-to)	81-83
Number of pages	3
Journal	Biochimica et Biophysica Acta - General Subjects
Volume	1670
Issue number	1
DOIs	https://doi.org/10.1016/j.bbagen.2003.10.014
Publication status	Published - 5 Jan 2004
Externally published	Yes

Keywords

EBV transformation
Lymphoblastoid cell line
Microsatellite
Population genetic
STR

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10.1016/j.bbagen.2003.10.014

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title = "Genetic instability in EBV-transformed lymphoblastoid cell lines",

abstract = "Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.",

keywords = "EBV transformation, Lymphoblastoid cell line, Microsatellite, Population genetic, STR",

author = "Aisha Mohyuddin and Qasim Ayub and Saima Siddiqi and Carvalho-Silva, {Denise R.} and Kehkashan Mazhar and Sadia Rehman and Sadaf Firasat and Ahsana Dar and Chris Tyler-Smith and Mehdi, {S. Qasim}",

note = "Funding Information: This work was supported by a Wellcome Trust Collaborative Research Initiative Grant to SQM. CT-S was supported by the CRC. We thank the donors for the samples used in this study.",

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doi = "10.1016/j.bbagen.2003.10.014",

language = "English",

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T1 - Genetic instability in EBV-transformed lymphoblastoid cell lines

AU - Mohyuddin, Aisha

AU - Ayub, Qasim

AU - Siddiqi, Saima

AU - Carvalho-Silva, Denise R.

AU - Mazhar, Kehkashan

AU - Rehman, Sadia

AU - Firasat, Sadaf

AU - Dar, Ahsana

AU - Tyler-Smith, Chris

AU - Mehdi, S. Qasim

N1 - Funding Information: This work was supported by a Wellcome Trust Collaborative Research Initiative Grant to SQM. CT-S was supported by the CRC. We thank the donors for the samples used in this study.

PY - 2004/1/5

Y1 - 2004/1/5

N2 - Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.

AB - Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.

KW - EBV transformation

KW - Lymphoblastoid cell line

KW - Microsatellite

KW - Population genetic

KW - STR

UR - http://www.scopus.com/inward/record.url?scp=9144264313&partnerID=8YFLogxK

U2 - 10.1016/j.bbagen.2003.10.014

DO - 10.1016/j.bbagen.2003.10.014

M3 - Short Survey

C2 - 14729144

AN - SCOPUS:9144264313

SN - 0304-4165

VL - 1670

SP - 81

EP - 83

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

IS - 1

ER -

Genetic instability in EBV-transformed lymphoblastoid cell lines

Abstract

Keywords

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