Genetic instability in EBV-transformed lymphoblastoid cell lines

Aisha Mohyuddin, Qasim Ayub, Saima Siddiqi, Denise R. Carvalho-Silva, Kehkashan Mazhar, Sadia Rehman, Sadaf Firasat, Ahsana Dar, Chris Tyler-Smith, S. Qasim Mehdi

Research output: Contribution to journalShort SurveyResearchpeer-review

23 Citations (Scopus)

Abstract

Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.

Original languageEnglish
Pages (from-to)81-83
Number of pages3
JournalBiochimica et Biophysica Acta - General Subjects
Volume1670
Issue number1
DOIs
Publication statusPublished - 5 Jan 2004
Externally publishedYes

Keywords

  • EBV transformation
  • Lymphoblastoid cell line
  • Microsatellite
  • Population genetic
  • STR

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