Genetic characterization of four stains Borrelia burgdorferi isolated in China

Xia Zeng; Shusheng Wang; Min Liu; Tao Zhang; Shengli Bi; Yongdong Zhou

Genetic characterization of four stains Borrelia burgdorferi isolated in China

Xia Zeng
, Shusheng Wang
, Min Liu
, Tao Zhang
, Shengli Bi
, Yongdong Zhou

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Objective: Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods: PCR technique was used to amplify the 5s-23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD-4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM-T Easy and sequenced. Results: The 5s-23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of > 99%. The four isolates had higher sequence identified with B. valaisiana than with other genomic groups. Conclusion: The four isolates most likely belong to Borrelia valaisiana genomic group.

Original language	English
Pages (from-to)	635-637
Number of pages	3
Journal	Chinese Journal of Microbiology and Immunology
Volume	22
Issue number	6
Publication status	Published - 30 Nov 2002
Externally published	Yes

Keywords

5s-23s rRNA (rrf-rrl) intergenic spacer DNA
Borrelia burgdorferi
Cloning
Sequence analysis

Cite this

@article{1f8cfd621ead48dbb4b088327a53f7c3,

title = "Genetic characterization of four stains Borrelia burgdorferi isolated in China",

abstract = "Objective: Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods: PCR technique was used to amplify the 5s-23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD-4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM-T Easy and sequenced. Results: The 5s-23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of > 99\%. The four isolates had higher sequence identified with B. valaisiana than with other genomic groups. Conclusion: The four isolates most likely belong to Borrelia valaisiana genomic group.",

keywords = "5s-23s rRNA (rrf-rrl) intergenic spacer DNA, Borrelia burgdorferi, Cloning, Sequence analysis",

author = "Xia Zeng and Shusheng Wang and Min Liu and Tao Zhang and Shengli Bi and Yongdong Zhou",

year = "2002",

month = nov,

day = "30",

language = "English",

volume = "22",

pages = "635--637",

journal = "Chinese Journal of Microbiology and Immunology",

issn = "0254-5101",

publisher = "Beijing Shengwu Zhipin Yanjiusuo",

number = "6",

}

TY - JOUR

T1 - Genetic characterization of four stains Borrelia burgdorferi isolated in China

AU - Zeng, Xia

AU - Wang, Shusheng

AU - Liu, Min

AU - Zhang, Tao

AU - Bi, Shengli

AU - Zhou, Yongdong

PY - 2002/11/30

Y1 - 2002/11/30

N2 - Objective: Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods: PCR technique was used to amplify the 5s-23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD-4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM-T Easy and sequenced. Results: The 5s-23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of > 99%. The four isolates had higher sequence identified with B. valaisiana than with other genomic groups. Conclusion: The four isolates most likely belong to Borrelia valaisiana genomic group.

AB - Objective: Genetic characterization of four strains of Borrelia burgdorferi isolated in China. Methods: PCR technique was used to amplify the 5s-23s rRNA intergenic spacer DNA from the whole cellular DNA of isolates GXLD-4,9,18 and Chang 14. The amplified products were then cloned into plasmid pGEM-T Easy and sequenced. Results: The 5s-23s rRNA/intergenic spacer DNA of the four isolates was 242bp, revealing a nucleotide sequence identity of > 99%. The four isolates had higher sequence identified with B. valaisiana than with other genomic groups. Conclusion: The four isolates most likely belong to Borrelia valaisiana genomic group.

KW - 5s-23s rRNA (rrf-rrl) intergenic spacer DNA

KW - Borrelia burgdorferi

KW - Cloning

KW - Sequence analysis

UR - https://www.scopus.com/pages/publications/0037202976

M3 - Article

AN - SCOPUS:0037202976

SN - 0254-5101

VL - 22

SP - 635

EP - 637

JO - Chinese Journal of Microbiology and Immunology

JF - Chinese Journal of Microbiology and Immunology

IS - 6

ER -

Genetic characterization of four stains Borrelia burgdorferi isolated in China

Abstract

Keywords

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