Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012

Celeste Donato; Le Thi Hoi; Nguyen Thi Hoa; Tran Mai Hoa; Le Van Duyet; Ta Thi Dieu Ngan; Nguyen Van Kinh; Nguyen Vu Trung; Dhanasekaran Vijaykrishna

doi:10.1016/j.virol.2016.04.026

Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012

Celeste Donato, Le Thi Hoi, Nguyen Thi Hoa, Tran Mai Hoa, Le Van Duyet, Ta Thi Dieu Ngan, Nguyen Van Kinh, Nguyen Vu Trung, Dhanasekaran Vijaykrishna

Research output: Contribution to journal › Article › Research › peer-review

30 Citations (Scopus)

Abstract

Background: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities.

Methods: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected.

Results: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases.

Conclusions: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.

Original language	English
Number of pages	9
Journal	Virology
Volume	495
DOIs	https://doi.org/10.1016/j.virol.2016.04.026
Publication status	Published - 1 Aug 2016
Externally published	Yes

Keywords

C4 subgenogroup
Enterovirus 71
Outbreak
Phylogenetics
Vietnam

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012",

abstract = "Background: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. Methods: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. Results: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. Conclusions: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.",

keywords = "C4 subgenogroup, Enterovirus 71, Outbreak, Phylogenetics, Vietnam",

author = "Celeste Donato and Hoi, {Le Thi} and Hoa, {Nguyen Thi} and Hoa, {Tran Mai} and {Van Duyet}, Le and {Dieu Ngan}, {Ta Thi} and {Van Kinh}, Nguyen and {Vu Trung}, Nguyen and Dhanasekaran Vijaykrishna",

year = "2016",

month = aug,

day = "1",

doi = "10.1016/j.virol.2016.04.026",

language = "English",

volume = "495",

journal = "Virology",

issn = "0042-6822",

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TY - JOUR

T1 - Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012

AU - Donato, Celeste

AU - Hoi, Le Thi

AU - Hoa, Nguyen Thi

AU - Hoa, Tran Mai

AU - Van Duyet, Le

AU - Dieu Ngan, Ta Thi

AU - Van Kinh, Nguyen

AU - Vu Trung, Nguyen

AU - Vijaykrishna, Dhanasekaran

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Background: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. Methods: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. Results: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. Conclusions: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.

AB - Background: Enterovirus 71 subgenogroup C4 caused the largest outbreak of Hand, Foot and Mouth Disease (HFMD) in Vietnam during 2011-2012, resulting in over 200,000 hospitalisations and 207 fatalities. Methods: A total of 1917 samples with adequate volume for RT-PCR analysis were collected from patients hospitalised with HFMD throughout Vietnam and 637 were positive for EV71. VP1 gene (n=87) and complete genome (n=9) sequencing was performed. Maximum-likelihood phylogenetic analysis was performed to characterise the B5, C4 and C5 strains detected. Results: Sequence analyses revealed that the dominant subgenogroup associated with the 2012 outbreak was C4, with B5 and C5 strains representing a small proportion of these cases. Conclusions: Numerous countries in the region including Malaysia, Taiwan and China have a large influence on strain diversity in Vietnam and understanding the transmission of EV71 throughout Southeast Asia is vital to inform preventative public health measures and vaccine development efforts.

KW - C4 subgenogroup

KW - Enterovirus 71

KW - Outbreak

KW - Phylogenetics

KW - Vietnam

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DO - 10.1016/j.virol.2016.04.026

M3 - Article

AN - SCOPUS:84966378367

SN - 0042-6822

VL - 495

JO - Virology

JF - Virology

ER -

Genetic characterization of Enterovirus 71 strains circulating in Vietnam in 2012

Abstract

Keywords

UN SDGs

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