Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium

Katherine J. Jeppe, Melissa E. Carew, Sara M. Long, Siu F. Lee, Vincent Pettigrove, Ary A. Hoffmann

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Freshwater invertebrates are often exposed to metal contamination, and changes in gene expression patterns can help understand mechanisms underlying toxicity and act as pollutant-specific biomarkers. In this study the expressions of genes involved in cysteine metabolism are characterized in the midge Chironomus tepperi during exposures to sublethal concentrations of cadmium and copper. These metals altered gene expression of the cysteine metabolism differently. Both metals decreased S-adenosylhomocysteine hydrolase expression and did not change the expression of S-adenosylmethionine synthetase. Cadmium exposure likely increased cystathionine production by up-regulating cystathionine-β-synthase (CβS) expression, while maintaining control level cysteine production via cystathionine-γ-lyase (CγL) expression. Conversely, copper down-regulated CβS expression and up-regulated CγL expression, which in turn could diminish cystathionine to favor cysteine production. Both metals up-regulated glutathione related expression (γ-glutamylcysteine synthase and glutathione synthetase). Only cadmium up-regulated metallothionein expression and glutathione S-transferase d1 expression was up-regulated only by copper exposure. These different transcription responses of genes involved in cysteine metabolism in C. tepperi point to metal-specific detoxification pathways and suggest that the transsulfuration pathway could provide biomarkers for identifying specific metals.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume162
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Cadmium
  • Chironomus
  • Copper
  • Cysteine
  • Gene expression
  • Transsulfuration

Cite this

Jeppe, Katherine J. ; Carew, Melissa E. ; Long, Sara M. ; Lee, Siu F. ; Pettigrove, Vincent ; Hoffmann, Ary A. / Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2014 ; Vol. 162, No. 1. pp. 1-6.
@article{945dd0efa5a647a69d64c11be754f1b5,
title = "Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium",
abstract = "Freshwater invertebrates are often exposed to metal contamination, and changes in gene expression patterns can help understand mechanisms underlying toxicity and act as pollutant-specific biomarkers. In this study the expressions of genes involved in cysteine metabolism are characterized in the midge Chironomus tepperi during exposures to sublethal concentrations of cadmium and copper. These metals altered gene expression of the cysteine metabolism differently. Both metals decreased S-adenosylhomocysteine hydrolase expression and did not change the expression of S-adenosylmethionine synthetase. Cadmium exposure likely increased cystathionine production by up-regulating cystathionine-β-synthase (CβS) expression, while maintaining control level cysteine production via cystathionine-γ-lyase (CγL) expression. Conversely, copper down-regulated CβS expression and up-regulated CγL expression, which in turn could diminish cystathionine to favor cysteine production. Both metals up-regulated glutathione related expression (γ-glutamylcysteine synthase and glutathione synthetase). Only cadmium up-regulated metallothionein expression and glutathione S-transferase d1 expression was up-regulated only by copper exposure. These different transcription responses of genes involved in cysteine metabolism in C. tepperi point to metal-specific detoxification pathways and suggest that the transsulfuration pathway could provide biomarkers for identifying specific metals.",
keywords = "Cadmium, Chironomus, Copper, Cysteine, Gene expression, Transsulfuration",
author = "Jeppe, {Katherine J.} and Carew, {Melissa E.} and Long, {Sara M.} and Lee, {Siu F.} and Vincent Pettigrove and Hoffmann, {Ary A.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.cbpc.2014.02.006",
language = "English",
volume = "162",
pages = "1--6",
journal = "Comparative Biochemistry and Physiology, Part C",
issn = "1532-0456",
publisher = "Elsevier",
number = "1",

}

Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium. / Jeppe, Katherine J.; Carew, Melissa E.; Long, Sara M.; Lee, Siu F.; Pettigrove, Vincent; Hoffmann, Ary A.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 162, No. 1, 01.01.2014, p. 1-6.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Genes involved in cysteine metabolism of Chironomus tepperi are regulated differently by copper and by cadmium

AU - Jeppe, Katherine J.

AU - Carew, Melissa E.

AU - Long, Sara M.

AU - Lee, Siu F.

AU - Pettigrove, Vincent

AU - Hoffmann, Ary A.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Freshwater invertebrates are often exposed to metal contamination, and changes in gene expression patterns can help understand mechanisms underlying toxicity and act as pollutant-specific biomarkers. In this study the expressions of genes involved in cysteine metabolism are characterized in the midge Chironomus tepperi during exposures to sublethal concentrations of cadmium and copper. These metals altered gene expression of the cysteine metabolism differently. Both metals decreased S-adenosylhomocysteine hydrolase expression and did not change the expression of S-adenosylmethionine synthetase. Cadmium exposure likely increased cystathionine production by up-regulating cystathionine-β-synthase (CβS) expression, while maintaining control level cysteine production via cystathionine-γ-lyase (CγL) expression. Conversely, copper down-regulated CβS expression and up-regulated CγL expression, which in turn could diminish cystathionine to favor cysteine production. Both metals up-regulated glutathione related expression (γ-glutamylcysteine synthase and glutathione synthetase). Only cadmium up-regulated metallothionein expression and glutathione S-transferase d1 expression was up-regulated only by copper exposure. These different transcription responses of genes involved in cysteine metabolism in C. tepperi point to metal-specific detoxification pathways and suggest that the transsulfuration pathway could provide biomarkers for identifying specific metals.

AB - Freshwater invertebrates are often exposed to metal contamination, and changes in gene expression patterns can help understand mechanisms underlying toxicity and act as pollutant-specific biomarkers. In this study the expressions of genes involved in cysteine metabolism are characterized in the midge Chironomus tepperi during exposures to sublethal concentrations of cadmium and copper. These metals altered gene expression of the cysteine metabolism differently. Both metals decreased S-adenosylhomocysteine hydrolase expression and did not change the expression of S-adenosylmethionine synthetase. Cadmium exposure likely increased cystathionine production by up-regulating cystathionine-β-synthase (CβS) expression, while maintaining control level cysteine production via cystathionine-γ-lyase (CγL) expression. Conversely, copper down-regulated CβS expression and up-regulated CγL expression, which in turn could diminish cystathionine to favor cysteine production. Both metals up-regulated glutathione related expression (γ-glutamylcysteine synthase and glutathione synthetase). Only cadmium up-regulated metallothionein expression and glutathione S-transferase d1 expression was up-regulated only by copper exposure. These different transcription responses of genes involved in cysteine metabolism in C. tepperi point to metal-specific detoxification pathways and suggest that the transsulfuration pathway could provide biomarkers for identifying specific metals.

KW - Cadmium

KW - Chironomus

KW - Copper

KW - Cysteine

KW - Gene expression

KW - Transsulfuration

UR - http://www.scopus.com/inward/record.url?scp=84896513326&partnerID=8YFLogxK

U2 - 10.1016/j.cbpc.2014.02.006

DO - 10.1016/j.cbpc.2014.02.006

M3 - Article

VL - 162

SP - 1

EP - 6

JO - Comparative Biochemistry and Physiology, Part C

JF - Comparative Biochemistry and Physiology, Part C

SN - 1532-0456

IS - 1

ER -