TY - JOUR
T1 - Genes associated with adult axon regeneration promoted by olfactory ensheathing cells: A new role for matrix metalloproteinase 2
AU - Pastrana, Erika
AU - Moreno-Flores, Maria Teresa
AU - Gurzov, Esteban
AU - Avila, Jesus
AU - Wadosell, Francisco
AU - Diaz-Nido, Javier
PY - 2006
Y1 - 2006
N2 - The molecular mechanisms used by olfactory ensheathing cells (OECs) to promote repair in the damaged adult mammalian CNS remain unknown. Thus, we used microarrays to analyze three OEC populations with different capacities to promote axonal regeneration in cultured rat retinal neurons. Gene expression in long-term cultured OECs that do not stimulate adult axonal outgrowth was compared with that of primary olfactory ensheathing cells and the immortalized OEC cell line TEG3. In this way, we identified a number of candidate genes that might play a role in promoting adult axonal regeneration. Among these genes, it was striking that both the matrix metalloproteinase 2 (MMP2) and an inhibitor of this protease were represented. The disruption of MMP2 activity in TEG3 cells impaired their capacity to trigger axon regeneration in cultured adult retinal neurons. Furthermore, the MMP2 protein was detected in grafts of OECs that elicited robust axonal regeneration in the injured spinal cord of adult rats in vivo. These data suggest that MMP2 does indeed participate in adult axonal regeneration induced by OECs.
AB - The molecular mechanisms used by olfactory ensheathing cells (OECs) to promote repair in the damaged adult mammalian CNS remain unknown. Thus, we used microarrays to analyze three OEC populations with different capacities to promote axonal regeneration in cultured rat retinal neurons. Gene expression in long-term cultured OECs that do not stimulate adult axonal outgrowth was compared with that of primary olfactory ensheathing cells and the immortalized OEC cell line TEG3. In this way, we identified a number of candidate genes that might play a role in promoting adult axonal regeneration. Among these genes, it was striking that both the matrix metalloproteinase 2 (MMP2) and an inhibitor of this protease were represented. The disruption of MMP2 activity in TEG3 cells impaired their capacity to trigger axon regeneration in cultured adult retinal neurons. Furthermore, the MMP2 protein was detected in grafts of OECs that elicited robust axonal regeneration in the injured spinal cord of adult rats in vivo. These data suggest that MMP2 does indeed participate in adult axonal regeneration induced by OECs.
UR - http://www.jneurosci.org/content/26/20/5347.full.pdf+html
U2 - 10.1523/JNEUROSCI.1111-06.2006
DO - 10.1523/JNEUROSCI.1111-06.2006
M3 - Article
SN - 0270-6474
VL - 26
SP - 5347
EP - 5359
JO - The Journal of Neuroscience
JF - The Journal of Neuroscience
IS - 20
ER -