Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease

Yujing Gao, Gabrielle R. Wilson, Kiymet Bozaoglu, Andrew G. Elefanty, Edouard G. Stanley, Mirella Dottori, Paul J. Lockhart

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Mutations in RAB39B are a known cause of X-linked early onset Parkinson's disease. Isogenic human embryonic stem cell lines carrying two independent deletions of RAB39B were generated using CRISPR/Cas9 genome editing tool. The deletions were confirmed by PCR and direct sequence analysis in two edited stem cell lines. Both cell lines showed pluripotency and displayed a normal karyotype. Further, they were able to form embryoid bodies in vitro, and express markers indicative of differentiation to the three germ layers.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalStem Cell Research
Volume28
DOIs
Publication statusPublished - 1 Apr 2018

Cite this

Gao, Yujing ; Wilson, Gabrielle R. ; Bozaoglu, Kiymet ; Elefanty, Andrew G. ; Stanley, Edouard G. ; Dottori, Mirella ; Lockhart, Paul J. / Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease. In: Stem Cell Research. 2018 ; Vol. 28. pp. 161-164.
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Generation of RAB39B knockout isogenic human embryonic stem cell lines to model RAB39B-mediated Parkinson's disease. / Gao, Yujing; Wilson, Gabrielle R.; Bozaoglu, Kiymet; Elefanty, Andrew G.; Stanley, Edouard G.; Dottori, Mirella; Lockhart, Paul J.

In: Stem Cell Research, Vol. 28, 01.04.2018, p. 161-164.

Research output: Contribution to journalArticleResearchpeer-review

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